Pig Vascular cell adhesion molecule 1,VCAM-1 ELISA Kit

產(chǎn)品詳情

文獻引用 (2) 說明書下載

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產(chǎn)品描述：

豬血管內(nèi)皮細胞粘附分子1(VCAM-1/CD106)酶聯(lián)免疫試劑盒（CSB-E06825p）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的VCAM1含量。VCAM1（血管細胞粘附分子1）是一種細胞表面糖蛋白，屬于免疫球蛋白超家族，主要表達于活化的內(nèi)皮細胞表面。它通過介導白細胞與內(nèi)皮細胞的粘附，在免疫反應和炎癥過程中發(fā)揮重要作用。研究顯示，VCAM1在多種疾病中扮演關(guān)鍵角色，如動脈粥樣硬化、炎癥性腸病和癌癥，其表達水平與疾病進展密切相關(guān)。試劑盒檢測范圍為15.6ng/mL-1000ng/mL，適用于研究豬心血管疾病模型中的內(nèi)皮細胞活化機制、炎癥性疾病病理進程評估，以及免疫調(diào)節(jié)藥物對血管內(nèi)皮功能影響的體外實驗，為探索VCAM - 1在血管相關(guān)疾病中的作用提供可靠工具本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
縮寫：

VCAM1/CD106
種屬：

Sus scrofa (Pig)
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

15.6ng/mL-1000ng/mL
靈敏度：

3.9 ng/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領(lǐng)域：

Signal Transduction
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of pig VCAM-1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	92
	Range %	85-94
1:2	Average %	94
	Range %	87-98
1:4	Average %	103
	Range %	97-107
1:8	Average %	101
	Range %	95-104

回收率：

The recovery of pig VCAM-1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	94	90-98
EDTA plasma (n=4)	89	83-94

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.