Pig Syndecan-1/CD138(SDC1) ELISA Kit

產品詳情

文獻引用 (3) 說明書下載

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產品描述：

豬Syndecan-1/CD138(SDC1)酶聯免疫試劑盒（CSB-E17413p）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的SDC1含量。豬Syndecan-1（CD138）是一種細胞表面硫酸乙酰肝素蛋白聚糖，參與細胞增殖、遷移和細胞基質相互作用。其表達異常與多種疾病相關，如癌癥和炎癥性疾病，研究其機制有助于開發新的治療策略。試劑盒檢測范圍為5 ng/mL-320 ng/mL，該產品可應用于豬模型研究中SDC1蛋白的表達分析，如評估炎癥性疾病進程、組織再生機制研究或作為特定病理狀態的潛在生物標志物探索，僅限科研用途。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
縮寫：

SDC1
Uniprot No.：

K7GNF5
種屬：

Sus scrofa (Pig)
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

5 ng/mL-320 ng/mL
靈敏度：

1.25 ng/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Signal Transduction
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of pig SDC1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	85
	Range %	80-90
1:2	Average %	93
	Range %	87-102
1:4	Average %	97
	Range %	92-102
1:8	Average %	91
	Range %	80-98

回收率：

The recovery of pig SDC1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	93	81-98
EDTA plasma (n=4)	98	90-105

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.