Pig Peptide YY(PYY) ELISA kit

產品詳情

文獻引用 (4) 說明書下載

Intra-assay Precision (Precision within an assay): CV%<15%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<15%

Three samples of known concentration were tested in twenty assays to assess.

產品描述：

豬多肽YY(PYY)酶聯免疫試劑盒（CSB-EL019128PI）為競爭法ELISA試劑盒，定量檢測血清、血漿樣本中的PYY含量。PYY即肽YY，是由腸道L細胞分泌的一種胃腸激素。它能減少胃腸道蠕動、抑制胃酸分泌等。其研究機制主要圍繞與受體結合發(fā)揮作用，在調節(jié)食欲、能量代謝等方面有重要意義，為肥胖、糖尿病等疾病的治療提供潛在靶點。試劑盒檢測范圍為62.5 pg/mL-1000 pg/mL，該產品適用于科研領域探索豬的消化生理機制、營養(yǎng)干預研究或代謝相關疾病模型的構建，例如肥胖、糖尿病等動物實驗中的激素動態(tài)監(jiān)測，為解析 PYY 在能量穩(wěn)態(tài)中的作用提供可靠工具。適用于基礎醫(yī)學、獸醫(yī)學及動物營養(yǎng)學等領域的科學研究。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
別名：

PYY ELISA Kit; Peptide YY ELISA Kit; PYY ELISA Kit; Peptide tyrosine tyrosine) [Cleaved into: Peptide YY(3-36 ELISA Kit; PYY-II)] ELISA Kit
縮寫：

PYY
Uniprot No.：

P68005
種屬：

Sus scrofa (Pig)
樣本類型：

serum, plasma
檢測范圍：

62.5 pg/mL-1000 pg/mL
靈敏度：

16.88 pg/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Neuroscience
測定原理：

quantitative
測定方法：

Competitive

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of pig PYY in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	92
	Range %	87-99
1:2	Average %	96
	Range %	91-100
1:4	Average %	94
	Range %	89-98
1:8	Average %	93
	Range %	87-99

回收率：

The recovery of pig PYY spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	96	91-101
EDTA plasma (n=4)	94	89-99

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

功能：

This gut peptide inhibits exocrine pancreatic secretion, has a vasoconstrictory action and inhibitis jejunal and colonic mobility.
亞細胞定位：

Secreted.
蛋白家族：

NPY family
數據庫鏈接：

STRING: 9823.ENSSSCP00000023751

UniGene: Ssc.53428