Pig Nuclear factor-kappa B,NF-κB ELISA Kit

產品詳情

說明書下載

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產品描述：

豬核因子-κB(NF-κB)酶聯免疫試劑盒（CSB-E13448p）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿、細胞裂解物樣本中的NF-κB含量。NF - κB是重要的轉錄因子，廣泛存在于多種細胞中。在細胞的存活、增殖、炎癥反應等生理病理過程中起到關鍵作用。研究機制上，它受多種信號通路調控，激活后能轉位至細胞核調控基因表達，其異常與腫瘤、炎癥等多種疾病相關。試劑盒檢測范圍為31.25 pg/mL-2000 pg/mL，該產品適用于動物模型構建、病原體感染機制研究、疫苗效力評估等科研領域本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
別名：

NF-kappa B,Nuclear factor-kappa B
縮寫：

NF-κB
種屬：

Sus scrofa (Pig)
樣本類型：

serum, plasma, tissue homogenates, cell lysates
檢測范圍：

31.25 pg/mL-2000 pg/mL
靈敏度：

7.81 pg/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Epigenetics and Nuclear Signaling
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of pig NF-κB in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	90
	Range %	87-95
1:2	Average %	103
	Range %	99-106
1:4	Average %	93
	Range %	87-98
1:8	Average %	91
	Range %	88-95

回收率：

The recovery of pig NF-κB spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	96	92-102
EDTA plasma (n=4)	90	86-93

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.