Pig Neuron-specific enolase,NSE ELISA Kit

產品詳情

文獻引用 (4) 說明書下載

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產品描述：

豬神經特異性烯醇化酶(NSE)酶聯免疫試劑盒（CSB-E14065p）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的NSE含量。試劑盒檢測范圍為0.78 ng/mL-50 ng/mL，適用于腦組織、外周神經等樣本的神經功能評估，兼容常規血清/血漿樣本（需離心去除沉淀后分裝凍存），可滿足神經退行性疾病模型構建、神經損傷修復研究以及神經內分泌相關疾病機制探索等科研需求，為動物實驗中的病理機制解析和藥物干預效果觀察提供可靠定量工具。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
縮寫：

NSE
種屬：

Sus scrofa (Pig)
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

0.78 ng/mL-50 ng/mL
靈敏度：

0.195 ng/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Cancer
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of pig NSE in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	91
	Range %	86-102
1:2	Average %	90
	Range %	85-99
1:4	Average %	95
	Range %	87-98
1:8	Average %	94
	Range %	86-104

回收率：

The recovery of pig NSE spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	84-102
EDTA plasma (n=4)	94	83-99

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.