Pig N-terminal pro-brain natriuretic peptide (NT-proBNP) ELISA kit

產(chǎn)品詳情

說明書下載

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產(chǎn)品描述：

豬N端前腦鈉素(NT-proBNP)酶聯(lián)免疫試劑盒（CSB-EQ027465PI）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的NT-proBNP含量。NT - proBNP即N末端B型利鈉肽原，是心室肌細(xì)胞分泌的BNP前體裂解產(chǎn)物，主要用于診斷心力衰竭和判斷預(yù)后。，心衰時(shí)心室壁張力增加，刺激NT - proBNP釋放，通過檢測其血漿水平變化為病情評(píng)估提供依據(jù)。試劑盒檢測范圍為7.8 pg/mL-500 pg/mL，豬心血管疾病模型研究、心臟病理機(jī)制探索、藥物干預(yù)效果評(píng)估、實(shí)驗(yàn)室開展豬心臟疾病模型構(gòu)建、心肌病機(jī)制研究、心血管藥物篩選、深入解析心臟疾病的分子調(diào)控機(jī)制本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
縮寫：

NT-proBNP
種屬：

Sus scrofa (Pig)
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

7.8 pg/mL-500 pg/mL
靈敏度：

1.95 pg/mL
反應(yīng)時(shí)間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領(lǐng)域：

Cardiovascular
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of pig NT-proBNP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	103
	Range %	96-108
1:2	Average %	99
	Range %	91-102
1:4	Average %	94
	Range %	86-98
1:8	Average %	98
	Range %	90-102

回收率：

The recovery of pig NT-proBNP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	105	99-109
EDTA plasma (n=4)	97	90-101

標(biāo)準(zhǔn)曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.