Pig Interleukin 1 receptor antagonist, IL-1ra ELISA kit

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產品詳情

產品描述：

豬白介素1受體拮抗劑(IL1Ra)酶聯免疫試劑盒（CSB-E04946p）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的IL1RN含量。IL1RN是重要的靶點。它是白細胞介素-1受體拮抗劑，可與IL-1競爭性結合IL-1受體，抑制IL-1的促炎作用。在研究中，聚焦其在炎癥性疾病如類風濕關節炎等發病機制中的作用，以探索新的治療策略。試劑盒檢測范圍為62.5 pg/mL-4000 pg/mL，該產品廣泛應用于科研領域，包括但不限于豬類炎癥性疾病模型的機制研究、免疫調節藥物開發中的靶標驗證，以及感染或應激條件下宿主免疫反應的動態監測，為探索IL - 1信號通路在動物生理病理過程中的作用提供可靠工具。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
別名：

IL1RN ELISA kit; IRAP1Interleukin-1 receptor antagonist protein ELISA kit; IL-1RN ELISA kit; IL-1ra ELISA kit; IRAP ELISA kit; IL1 inhibitor ELISA kit
縮寫：

IL1RN
Uniprot No.：

Q29056
種屬：

Sus scrofa (Pig)
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

62.5 pg/mL-4000 pg/mL
靈敏度：

15.6 pg/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Others
測定原理：

quantitative
測定方法：

Sandwich

精密度：

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of pig IL-1ra in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	106
	Range %	93-110
1:2	Average %	101
	Range %	91-105
1:4	Average %	97
	Range %	87-101
1:8	Average %	91
	Range %	84-95

回收率：

The recovery of pig IL-1ra spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	92	85-96
EDTA plasma (n=4)	108	98-112

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

功能：

Inhibits the activity of interleukin-1 by binding to receptor IL1R1 and preventing its association with the coreceptor IL1RAP for signaling. Has no interleukin-1 like activity.
基因功能參考文獻：
1. This study identified IL-1Ra as an early immunomodulatory mediator that could be involved in the immunopathogenesis of porcine reproductive and respiratory syndrome virus infections. PMID: 27902420
亞細胞定位：

Secreted.
蛋白家族：

IL-1 family
數據庫鏈接：

UniGene: Ssc.16250