Pig Interleukin 1α,IL-1α ELISA kit

產品詳情

文獻引用 (1) 說明書下載

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產品描述：

豬白介素1α(IL-1α)酶聯免疫試劑盒（CSB-E09725p）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織培養上清液、組織勻漿樣本中的IL1A含量。IL1A是重要的細胞因子，在炎癥反應等生理病理過程中發揮關鍵作用。其與多種炎癥及免疫相關疾病關聯。研究機制上，它主要通過與相應受體結合，激活下游信號通路，從而調節免疫細胞功能、介導炎癥反應，是當前熱門研究靶點。試劑盒檢測范圍為25 pg/mL-1600 pg/mL，可為豬類動物模型中的炎癥機制研究、疫苗開發評估、疾病進程監測等科研項目提供可靠數據支持，例如在豬繁殖與呼吸綜合征、豬流行性腹瀉等感染性疾病模型中定量分析IL - 1α的動態變化，或在免疫調控實驗中評估細胞因子網絡的作用機制。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
別名：

IL1A ELISA Kit; Interleukin-1 alpha ELISA Kit; IL-1 alpha ELISA Kit
縮寫：

IL1A
Uniprot No.：

P18430
種屬：

Sus scrofa (Pig)
樣本類型：

serum, plasma, cell culture supernates, tissue homogenates
檢測范圍：

25 pg/mL-1600 pg/mL
靈敏度：

6.25 pg/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Immunology
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of pig IL-1α in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	87
	Range %	82-93
1:2	Average %	104
	Range %	100-108
1:4	Average %	91
	Range %	85-97
1:8	Average %	87
	Range %	83-92

回收率：

The recovery of pig IL-1α spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	99	95-103
EDTA plasma (n=4)	95	90-100

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

功能：

Produced by activated macrophages, IL-1 stimulates thymocyte proliferation by inducing IL-2 release, B-cell maturation and proliferation, and fibroblast growth factor activity. IL-1 proteins are involved in the inflammatory response, being identified as endogenous pyrogens, and are reported to stimulate the release of prostaglandin and collagenase from synovial cells.
基因功能參考文獻：
1. Constitutive suppression of IL-1alpha maintains quiescence of endothelium and that terminal complement complexes remove that suppression, allowing IL-1alpha transcription and, ultimately, activation of endothelium to proceed. PMID: 18292602
亞細胞定位：

Cytoplasm. Secreted.
蛋白家族：

IL-1 family
數據庫鏈接：

KEGG: ssc:397094

STRING: 9823.ENSSSCP00000008638

UniGene: Ssc.113