Pig Haptoglobin,Hpt/HP ELISA Kit

Pig Haptoglobin,Hpt/HP ELISA Kit

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文獻引用 (5) 說明書下載

中文名稱：

豬結合珠蛋白/觸珠蛋白(Hpt/HP)酶聯免疫試劑盒
貨號：

CSB-E13424p
規格：

96T/48T
價格：

￥4200/￥3000
其他：

CUSABIO Wins 2024 Bioz Rapid Star? Award in the ELISA category

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產品描述：

豬結合珠蛋白/觸珠蛋白(Hpt/HP)酶聯免疫試劑盒（CSB-E13424p）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的HP含量。試劑盒檢測范圍為1.56 μg/mL-100 μg/mL，其應用場景涵蓋動物醫學研究中的炎癥標志物動態監測、疾病模型建立效果評估，以及畜牧養殖中豬群健康狀態的分子水平研究，為探索Hpt/HP在病理生理過程中的作用提供可靠工具。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
別名：

HP ELISA Kit; Haptoglobin [Cleaved into: Haptoglobin alpha chain; Haptoglobin beta chain] ELISA Kit
縮寫：

HP
Uniprot No.：

Q8SPS7
種屬：

Sus scrofa (Pig)
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

1.56 μg/mL-100 μg/mL
靈敏度：

0.39 μg/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Cardiovascular
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of pig Hpt/HP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:20	Average %	93
	Range %	89-98
1:40	Average %	98
	Range %	93-103
1:80	Average %	95
	Range %	89-98
1:160	Average %	94
	Range %	87-98

回收率：

The recovery of pig Hpt/HP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	98	94-102
EDTA plasma (n=4)	87	82-94

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ug/ml	OD1	OD2	Average	Corrected
100	1.812	1.724	1.768	1.625
50	1.145	1.212	1.179	1.036
25	0.715	0.724	0.720	0.577
12.5	0.451	0.438	0.445	0.302
6.25	0.312	0.322	0.317	0.174
3.12	0.255	0.242	0.249	0.106
1.56	0.196	0.190	0.193	0.050
0	0.142	0.144	0.143

數據處理：

ELISA操作視頻及標曲繪制軟件下載
貨期：

3-5 working days

Hydroxy-Selenomethionine Supplementation During Gestation and Lactation Improve Reproduction of Sows by Enhancing the Antioxidant Capacity and Immunity Under Heat Stress Conditions J Wang, H Sun, Z Peng, SQ Wang, YQ Yan, WC Luo,Antioxidants,2025
Effects of maternal contact and positive human contact during lactation on pork quality: Positive human contact to piglets during lactation improves pork loin muscle pH K Tomas, J Savaglia, RJE Hewitt, KJ Plush,Meat Science,2025
Early human contact and housing for pigs-part 2: resilience to routine husbandry practices ME Lucas,Animal,2024
Maternal Contact and Positive Human Interactions During Lactation Impact on Pig Stress Resilience Post-Weaning K Tomas,/,/
Maternal contact and positive human interactions during lactation impacts piglet performance and behaviour during lactation K Tomas,/,2024

功能：

As a result of hemolysis, hemoglobin is found to accumulate in the kidney and is secreted in the urine. Haptoglobin captures, and combines with free plasma hemoglobin to allow hepatic recycling of heme iron and to prevent kidney damage. Haptoglobin also acts as an antioxidant, has antibacterial activity and plays a role in modulating many aspects of the acute phase response. Hemoglobin/haptoglobin complexes are rapidly cleared by the macrophage CD163 scavenger receptor expressed on the surface of liver Kupfer cells through an endocytic lysosomal degradation pathway.
基因功能參考文獻：
1. crystal structure of the dimeric porcine haptoglobin-haemoglobin complex determined at 2.9 A resolution PMID: 22922649
2. This protein is elevated in porcine postweaning multisystemic wasting syndrome. PMID: 15210076
3. haptoglobin has a role in oxidative status in piglets undergoing weaning-induced stress PMID: 16438801
4. This study suggested closer links between the release of haptoglobin, Pig-MAP and monocytes compared to the release of AGP, SAA and transthyretin. PMID: 17629575
5. The haptoglobin was down-regulated with SNPs PMID: 18777092
6. Presence of haptoglobin alpha 1S subunit is a reponse to respiratory syndrome virus infection. PMID: 18931071
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亞細胞定位：

Secreted.
蛋白家族：

Peptidase S1 family
組織特異性：

Expressed by the liver and secreted in plasma.
數據庫鏈接：

KEGG: ssc:397061

STRING: 9823.ENSSSCP00000002967

UniGene: Ssc.37

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.