Pig Corticoliberin(CRH) ELISA kit

Pig Corticoliberin(CRH) ELISA kit

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中文名稱：

豬促腎上腺皮質(zhì)素釋放素(CRH)酶聯(lián)免疫試劑盒
貨號：

CSB-EL005963PI
規(guī)格：

96T/48T
價(jià)格：

￥4200/￥3000
其他：

CUSABIO Wins 2024 Bioz Rapid Star? Award in the ELISA category

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產(chǎn)品描述：

豬促腎上腺皮質(zhì)素釋放素(CRH)酶聯(lián)免疫試劑盒（CSB-EL005963PI）為競爭法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的CRH含量。CRH（促腎上腺皮質(zhì)激素釋放激素）是一種重要的神經(jīng)調(diào)節(jié)物質(zhì)，主要作用是促進(jìn)腺垂體合成與釋放促腎上腺皮質(zhì)激素（ACTH）。近年來，研究發(fā)現(xiàn)CRH在慢性疼痛誘發(fā)的焦慮和抑郁中發(fā)揮關(guān)鍵作用，其調(diào)控痛相關(guān)抑郁的神經(jīng)環(huán)路機(jī)制已得到揭示。試劑盒檢測范圍為100 pg/mL-1600 pg/mL，適用于基礎(chǔ)科研領(lǐng)域，例如探究豬在環(huán)境壓力、代謝異常或特定病理狀態(tài)下CRH的表達(dá)規(guī)律，亦可應(yīng)用于神經(jīng)生物學(xué)、動物生理學(xué)及藥理學(xué)研究中激素調(diào)控網(wǎng)絡(luò)的解析本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
別名：

CRHCorticoliberin ELISA kit; Corticotropin-releasing factor ELISA kit; CRF ELISA kit; Corticotropin-releasing hormone ELISA kit
縮寫：

CRH
Uniprot No.：

P06296
種屬：

Sus scrofa (Pig)
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

100 pg/mL-1600 pg/mL
靈敏度：

100 pg/mL
反應(yīng)時(shí)間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領(lǐng)域：

Neuroscience
測定原理：

quantitative
測定方法：

Competitive

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of pig CRH in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	90
	Range %	85-95
1:2	Average %	95
	Range %	92-101
1:4	Average %	82
	Range %	80-83
1:8	Average %	88
	Range %	85-92

回收率：

The recovery of pig CRH spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	93	87-97
EDTA plasma (n=4)	94	90-98

標(biāo)準(zhǔn)曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average
1600	0.196	0.184	0.190
800	0.322	0.343	0.333
400	0.516	0.510	0.513
200	1.037	1.100	1.069
100	2.158	2.323	2.241

數(shù)據(jù)處理：

ELISA操作視頻及標(biāo)曲繪制軟件下載
貨期：

3-5 working days

Effects of high ambient temperature on meat quality, serum hormone concentrations, and gene expression in the longissimus dorsi muscle of finishing pigs Shi Z B.et al,Animal Production Science, ,2016

功能：

Hormone regulating the release of corticotropin from pituitary gland. Induces NLRP6 in intestinal epithelial cells, hence may influence gut microbiota profile.
基因功能參考文獻(xiàn)：
1. Evidence is provided for porcine corticotropin releasing hormone (CRH) as a quantitative trait locus (QTL) affecting growth and body composition. (corticotropin releasing hormone; CRH; CRF) PMID: 16483545
2. CRH inhibits local progesterone production from luteal cells in swine corpus lutem. PMID: 20431270
亞細(xì)胞定位：

Secreted.
蛋白家族：

Sauvagine/corticotropin-releasing factor/urotensin I family
組織特異性：

Produced by the hypothalamus.
數(shù)據(jù)庫鏈接：

KEGG: ssc:100127468

STRING: 9823.ENSSSCP00000006626

UniGene: Ssc.69887

Intra-assay Precision (Precision within an assay): CV%<15%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<15%

Three samples of known concentration were tested in twenty assays to assess.