Pig Colipase(CLPS) ELISA kit

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產(chǎn)品詳情

產(chǎn)品描述：

豬輔脂酶(CLPS)酶聯(lián)免疫試劑盒（CSB-EL005578PI）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的CLPS含量。試劑盒檢測范圍為15.6 mU/mL-1000 mU/mL，該產(chǎn)品適用于動物營養(yǎng)代謝研究、消化系統(tǒng)疾病機制探索、飼料添加劑功效評估等科研場景，特別在豬模型研究中可量化評估胰腺功能或脂肪吸收狀態(tài)本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
別名：

CLPS ELISA Kit; Colipase ELISA Kit; Procolipase II ELISA Kit
縮寫：

CLPS
Uniprot No.：

P02703
種屬：

Sus scrofa (Pig)
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

15.6 mU/mL-1000 mU/mL
靈敏度：

3.9 mU/mL
反應(yīng)時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領(lǐng)域：

Signal Transduction
測定原理：

quantitative
測定方法：

Sandwich

精密度：

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of pig CLPS in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	87
	Range %	82-92
1:2	Average %	99
	Range %	94-103
1:4	Average %	103
	Range %	98-107
1:8	Average %	95
	Range %	90-101

回收率：

The recovery of pig CLPS spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	90	85-105
EDTA plasma (n=4)	104	88-111

標(biāo)準(zhǔn)曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

功能：

Colipase is a cofactor of pancreatic lipase. It allows the lipase to anchor itself to the lipid-water interface. Without colipase the enzyme is washed off by bile salts, which have an inhibitory effect on the lipase.; Enterostatin has a biological activity as a satiety signal.
基因功能參考文獻：
1. isotropic orientation of colipase which could explain its incapacity to reverse the inhibitory effects of these lipids on the lipase activity PMID: 18052211
2. Analysis of structures of NMR colipase models and simulations of their interactions with various lipid aggregates, lipid droplet, and bile salt micelle, were carried out to determine and to map the lipid binding sites on colipase. PMID: 18506778
亞細胞定位：

Secreted.
蛋白家族：

Colipase family
組織特異性：

Expressed by the pancreas.
數(shù)據(jù)庫鏈接：

KEGG: ssc:397407

STRING: 9823.ENSSSCP00000001686

UniGene: Ssc.514