Pig Angiogenin(ANG) ELISA kit

產(chǎn)品詳情

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產(chǎn)品描述：

豬血管生長素(ANG)酶聯(lián)免疫試劑盒（CSB-EL001703PI）為競爭法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的ANG含量。豬血管生長素（ANG）是RNA酶A超家族成員，具有刺激血管生長的功能。研究發(fā)現(xiàn)，ANG基因可能與宿主防御有關(guān)，其變異較大，對系統(tǒng)先天免疫很重要。ANG蛋白對多種病原微生物具有殺傷力，在抗病育種和疾病治療中具有潛在應(yīng)用價值。試劑盒檢測范圍為94 pg/mL-6000 pg/mL，適用于心血管疾病模型研究、再生醫(yī)學(xué)實驗、炎癥相關(guān)血管病理分析等科研場景，特別在豬源樣本的血管生成機(jī)制探索中具有重要應(yīng)用價值本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
別名：

ANGAngiogenin ELISA kit; EC 3.1.27.- ELISA kit; Ribonuclease 5 ELISA kit; RNase 5 ELISA kit
縮寫：

ANG
Uniprot No.：

P31346
種屬：

Sus scrofa (Pig)
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

94 pg/mL-6000 pg/mL
靈敏度：

47 pg/mL
反應(yīng)時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領(lǐng)域：

Cardiovascular
測定原理：

quantitative
測定方法：

Competitive

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of pig ANG in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:100	Average %	94
	Range %	90-98
1:200	Average %	96
	Range %	92-99
1:400	Average %	103
	Range %	97-107
1:800	Average %	84
	Range %	80-88

回收率：

The recovery of pig ANG spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	94	90-98
EDTA plasma (n=4)	98	94-102

標(biāo)準(zhǔn)曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

功能：

Binds to actin on the surface of endothelial cells; once bound, angiogenin is endocytosed and translocated to the nucleus. Stimulates ribosomal RNA synthesis including that containing the initiation site sequences of 45S rRNA. Cleaves tRNA within anticodon loops to produce tRNA-derived stress-induced fragments (tiRNAs) which inhibit protein synthesis and triggers the assembly of stress granules (SGs). Angiogenin induces vascularization of normal and malignant tissues. Angiogenic activity is regulated by interaction with RNH1 in vivo.
亞細(xì)胞定位：

Cytoplasmic vesicle, secretory vesicle lumen. Secreted. Nucleus, nucleolus.
蛋白家族：

Pancreatic ribonuclease family
數(shù)據(jù)庫鏈接：

STRING: 9823.ENSSSCP00000020122

UniGene: Ssc.11079