Mouse β-defensins 1 ELISA Kit

Mouse β-defensins 1 ELISA Kit

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中文名稱：

小鼠β-防御素1酶聯免疫試劑盒
貨號：

CSB-E14188m
規格：

96T/48T
價格：

￥3600/￥2500
其他：

CUSABIO Wins 2024 Bioz Rapid Star? Award in the ELISA category

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小鼠β-防御素1酶聯免疫試劑盒（CSB-E14188m）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織培養上清液、組織勻漿樣本中的DEFB1含量。DEFB1（防御素β1）是一種抗菌肽，由中性粒細胞產生，與上皮表面對抗微生物定植的抵抗力有關。其基因表達與多種疾病相關，如齲齒、牙周炎和囊性纖維化。研究顯示，DEFB1在男性生育過程中發揮促進精子活力和殺菌的雙重作用，其缺陷可能導致弱精子癥和精液白細胞增多癥。試劑盒檢測范圍為15.6 pg/mL-1000 pg/mL，適用于科研場景中探究β-防御素1在感染性疾病模型、炎癥反應機制或免疫調節研究中的表達變化，例如評估病原體刺激下組織局部防御素分泌動態、分析免疫相關基因敲除動物模型的黏膜屏障功能，或體外研究細胞因子對防御素表達的調控作用本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
別名：

Defb1Beta-defensin 1 ELISA kit; BD-1 ELISA kit; mBD-1 ELISA kit; Defensin ELISA kit; beta 1 ELISA kit
縮寫：

DEFB1
Uniprot No.：

P56386
種屬：

Mus musculus (Mouse)
樣本類型：

serum, plasma, cell culture supernates, tissue homogenates
檢測范圍：

15.6 pg/mL-1000 pg/mL
靈敏度：

3.9 pg/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Microbiology
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of mouse β-defensins 1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:5	Average %	93
	Range %	89-106
1:10	Average %	92
	Range %	83-100
1:20	Average %	95
	Range %	85-110
1:40	Average %	94
	Range %	89-103

回收率：

The recovery of mouse β-defensins 1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	97	91-103
EDTA plasma (n=4)	100	95-105

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
1000	2.184	2.052	2.118	1.936
500	1.363	1.377	1.370	1.188
250	0.890	0.861	0.876	0.694
125	0.517	0.482	0.500	0.318
62.5	0.375	0.364	0.370	0.188
31.2	0.286	0.267	0.277	0.095
15.6	0.231	0.225	0.228	0.046
0	0.184	0.179	0.182

數據處理：

ELISA操作視頻及標曲繪制軟件下載
貨期：

3-5 working days

Dendrobium huoshanense polysaccharide regionally regulates intestinal mucosal barrier function and intestinal microbiota in mice Song-ZiXie, et al,Carbohydrate Polymers,2018

功能：

Has bactericidal activity. May act as a ligand for C-C chemokine receptor CCR6. Positively regulates the sperm motility and bactericidal activity in a CCR6-dependent manner. Binds to CCR6 and triggers Ca2+ mobilization in the sperm which is important for its motility.
基因功能參考文獻：
1. Fenofibrate and gemfibrozil inhibit LPS-induced inflammatory activation of macrophages via PPARalpha/beta-defensin 1/TLR4 pathway. PMID: 25881202
2. these studies demonstrate a role for the murine beta-defensin 1 peptide in early control of Candida infection in a murine model of mucosal candidiasis PMID: 25595775
3. Akt1 is involved in the regulation of defensin expression and the innate immune response important for bacterial clearance PMID: 21559496
4. Histopathology showed a greater inflammatory influx in the lungs of mBD-1((-/-)) mice at Day 3 postinfection compared with WT C57BL/6 mice PMID: 21551252
5. The decreased production of antimicrobial peptides by burn-site epidermal keratinocytes influenced by Gr-1(+)CD11b(+) cells was shown to be restored by glycyrrhizin. PMID: 19843573
6. elimination of mBD-1 results in a defect in the ability of the host to clear H. influenzae from the lung, which is most consistent with this peptide functioning as an antibiotic at the airway surface. PMID: 12010999
7. Murine beta-defensins-1 and -4 were present in newborn skin. PMID: 12612195
8. The expression of BD1 was significantly elevated in the lungs of cigarette smoke-exposed mice compared with air-exposed mice. PMID: 18699806
9. Although both murine beta-defensin (mBD)-1 and mBD2 are constitutively expressed in normal BALB/c and C57BL/6 corneas, mBD-1 is not required for host resistance against Pseudomonas aeruginosa-induced corneal infection. PMID: 19155510
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亞細胞定位：

Secreted. Membrane.
蛋白家族：

Beta-defensin family
組織特異性：

Detected in kidney.
數據庫鏈接：

KEGG: mmu:13214

STRING: 10090.ENSMUSP00000051754

UniGene: Mm.431316

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.