Mouse trypsin ELISA Kit

產(chǎn)品詳情

文獻引用 (1) 說明書下載

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產(chǎn)品描述：

小鼠胰蛋白酶(trypsin)酶聯(lián)免疫試劑盒（CSB-E09900m）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織培養(yǎng)上清液、組織勻漿樣本中的trypsin含量。胰蛋白酶是一種絲氨酸蛋白酶，主要在消化系統(tǒng)中將蛋白質(zhì)分解為肽和氨基酸。它通過特異性切割賴氨酸和精氨酸殘基的肽鍵來發(fā)揮作用，是蛋白質(zhì)消化和蛋白質(zhì)組學(xué)研究中不可或缺的工具酶。試劑盒檢測范圍為1.56 ng/mL-100 ng/mL，適用于科研領(lǐng)域中對胰蛋白酶表達水平的定量分析，例如研究胰腺疾病模型中的酶活性變化、評估細(xì)胞培養(yǎng)體系中胰蛋白酶的釋放規(guī)律，或分析組織樣本在特定生理病理條件下的蛋白酶動態(tài)。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
縮寫：

trypsin
種屬：

Mus musculus (Mouse)
樣本類型：

serum, plasma, cell culture supernates, tissue homogenates
檢測范圍：

1.56 ng/mL-100 ng/mL
靈敏度：

0.39 ng/mL
反應(yīng)時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領(lǐng)域：

Signal Transduction
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of mouse trypsin in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	92
	Range %	89-95
1:2	Average %	103
	Range %	99-107
1:4	Average %	92
	Range %	87-96
1:8	Average %	98
	Range %	94-101

回收率：

The recovery of mouse trypsin spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	92	87-96
EDTA plasma (n=4)	95	89-98

標(biāo)準(zhǔn)曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.