Mouse prostacycline,PGI2 ELISA Kit

產品詳情

文獻引用 (2) 說明書下載

Intra-assay Precision (Precision within an assay): CV%<15%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<15%

Three samples of known concentration were tested in twenty assays to assess.

產品描述：

小鼠前列環素(PGI2)酶聯免疫試劑盒（CSB-E13698m）為競爭法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的PGI2含量。PGI2即前列環素，是一種具有強大血管舒張和血小板聚集抑制作用的內源性前列腺素。其合成主要由血管內皮細胞完成。研究機制上，它通過與特定受體結合，激活腺苷酸環化酶，升高細胞內cAMP水平，從而發揮生物學效應。試劑盒檢測范圍為25 pg/mL-1000 pg/mL，該產品適用于心血管疾病模型、抗血栓藥物機制、內皮功能評估等科研場景，尤其適合探究PGI2在炎癥微環境、血管舒縮調節及組織損傷修復中的分子作用，為相關基礎研究提供精準的定量數據支持。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
縮寫：

PGI2
種屬：

Mus musculus (Mouse)
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

25 pg/mL-1000 pg/mL
靈敏度：

25 pg/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Biochemicals
測定原理：

quantitative
測定方法：

Competitive

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of mouse PGI2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	95
	Range %	86-108
1:2	Average %	88
	Range %	82-100
1:4	Average %	95
	Range %	85-105
1:8	Average %	93
	Range %	84-100

回收率：

The recovery of mouse PGI2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	97	91-108
EDTA plasma (n=4)	96	87-105

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.