Mouse pro interleukin 1 beta (pro-IL1B) ELISA Kit

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產品詳情

產品描述：

小鼠白介素1β前體(pro-IL1B)酶聯免疫試劑盒（CSB-ET011614MO）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織培養上清液、組織勻漿樣本中的pro-IL1B含量。pro-IL1B，即前白細胞介素-1β，是一種無活性的前體蛋白，由pro-Il-1b基因編碼。它在炎癥刺激下被激活，裂解為具有生物活性的IL-1β，參與炎癥和免疫反應。研究顯示，pro-IL1B在腫瘤發生發展中扮演重要角色，通過促進炎癥反應和腫瘤血管生成，影響腫瘤的侵襲和轉移。試劑盒檢測范圍為6.25 pg/mL-400 pg/mL，適用于體外炎癥模型構建、免疫調控機制研究、藥物抗炎效果評估等科研領域。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
縮寫：

pro-IL1B
種屬：

Mus musculus (Mouse)
樣本類型：

serum, plasma, cell culture supernates, tissue homogenates
檢測范圍：

6.25 pg/mL-400 pg/mL
靈敏度：

1.56 pg/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Immunology
測定原理：

quantitative
測定方法：

Sandwich

精密度：

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of mouse pro-IL1B in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	106
	Range %	101-113
1:2	Average %	87
	Range %	82-91
1:4	Average %	94
	Range %	90-99
1:8	Average %	85
	Range %	80-90

回收率：

The recovery of mouse pro-IL1B spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	96	91-100
EDTA plasma (n=4)	91	85-97

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.