Mouse matrix metalloproteinase 10,MMP-10 ELISA kit

說明書下載

中文名稱：

小鼠基質金屬蛋白酶10(MMP-10)酶聯免疫試劑盒
貨號：

CSB-E07415m
規格：

96T/48T
價格：

￥3200/￥2500
其他：

CUSABIO Wins 2024 Bioz Rapid Star? Award in the ELISA category

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產品描述：

小鼠基質金屬蛋白酶10(MMP-10)酶聯免疫試劑盒（CSB-E07415m）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的MMP10含量。MMP10 即基質金屬蛋白酶 10，在細胞外基質降解與重塑中發揮關鍵作用。其高表達與腫瘤的侵襲、轉移及血管生成相關。研究機制聚焦于它對基質成分的酶解作用，以及與細胞信號通路的交互，以探索其在疾病診療中的應用。試劑盒檢測范圍為15.6 pg/mL-1000 pg/mL，能夠支持研究者在小鼠模型的組織纖維化、腫瘤侵襲轉移或炎癥性疾病等機制研究中，動態監測MMP - 10的表達變化，為探索其在病理生理過程中的功能提供可靠工具。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
別名：

Mmp10 ELISA Kit; Stromelysin-2 ELISA Kit; SL-2 ELISA Kit; EC 3.4.24.22 ELISA Kit; Matrix metalloproteinase-10 ELISA Kit; MMP-10 ELISA Kit; Transin-2 ELISA Kit
縮寫：

MMP10
Uniprot No.：

O55123
種屬：

Mus musculus (Mouse)
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

15.6 pg/mL-1000 pg/mL
靈敏度：

3.9 pg/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Cancer
測定原理：

quantitative
測定方法：

Sandwich

精密度：

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of mouse MMP-10 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	105
	Range %	101-114
1:2	Average %	91
	Range %	86-95
1:4	Average %	98
	Range %	94-102
1:8	Average %	87
	Range %	82-93

回收率：

The recovery of mouse MMP-10 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	92	88-96
EDTA plasma (n=4)	84	80-88

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
1000	2.517	2.401	2.459	2.246
500	1.791	1.736	1.764	1.551
250	1.105	1.058	1.082	0.869
125	0.683	0.662	0.673	0.460
62.5	0.412	0.409	0.411	0.198
31.25	0.329	0.340	0.335	0.122
15.6	0.268	0.273	0.271	0.058
0	0.210	0.215	0.213

數據處理：

ELISA操作視頻及標曲繪制軟件下載
貨期：

3-5 working days

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功能：

Can degrade fibronectin, gelatins of type I, III, IV, and V; weakly collagens III, IV, and V. Activates procollagenase.
基因功能參考文獻：
1. These results indicate that MMP10 serves a beneficial role in response to acute infection by moderating the proinflammatory response of resident and infiltrating macrophages. PMID: 27316687
2. MMP-10 facilitates the clearance of multiwalled carbon nanotubesand moderates the pro-inflammatory response of exposed alveolar and infiltrated macrophages. PMID: 28223796
3. crosstalk between MMP10 and the CXCR4/SDF1 axis contributes to hepatocarcinogenesis PMID: 25808184
4. Matrix metalloproteinase-10 expression is induced during hepatic injury and plays a fundamental role in liver tissue repair. PMID: 24119197
5. our findings support a model in which MMP-10 activity modulates CXCR4/SDF1 signaling, which is essential for efficient skeletal muscle regeneration. PMID: 24548137
6. Dissection of the matrix metalloproteinase 10 (MMP10) substrate degradome in fibroblast secretomes. PMID: 24281761
7. Thus, our findings indicate that MMP-10 is critical for skeletal muscle maintenance and regeneration during injury and disease. PMID: 24123596
8. MMP10 promotes macrophage movement PMID: 23691065
9. Assessed MMP-10's role in a murine model of colonic tissue damage induced by dextran sulfate sodium(DSS) treatment, and conclude MMP10 is required for resolution of DSS-induced colonic damage, and in its absence, chronic inflammation and dysplasia occurs. PMID: 23044923
10. Mmp10 is required for maintenance of a highly tumorigenic, cancer-initiating, metastatic stem-like cell population in lung cancer. PMID: 22545096
11. MMP-10 was capable of enhancing tissue plasminogen activator-induced fibrinolysis via a thrombin-activatable fibrinolysis inhibitor inactivation-mediated mechanism. PMID: 22104553
12. Data show that S100a9, Mmp10 and Ang2 were among the most downregulated genes, whereas Per3, Tef and Prdx6 were significantly upregulated in the DMH + Calcium group. PMID: 21857934
13. These findings support the hypothesis that CHF1/Hey2 is an important regulator of MMP10 expression. PMID: 22079635
14. Data demonstrate induction of MMP-10 by VEGF in HUVEC and support an angiogenic role for MMP-10 in response to VEGF stimulation in vitro and in vivo. PMID: 20432469
15. Results suggest that MMP-10 may be important in the initial stages of squamous cell cancer progression and induced in the stroma relating to the general host-response reaction to skin cancer. PMID: 19601983
16. Mice injected with lymphoma cells constitutively expressing MMP10 develop thymic lymphoma more rapidly than those injected with control lymphoma cells. PMID: 15356104
17. a tightly regulated expression level of stromelysin-2 is required for limited matrix degradation at the wound site PMID: 15371548
18. These data indicate that MMP-10 does not significantly contribute to adipose tissue development and associated angiogenesis in a mouse model of nutritionally induced obesity. PMID: 19732744
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亞細胞定位：

Secreted, extracellular space, extracellular matrix.
蛋白家族：

Peptidase M10A family
組織特異性：

Expressed in small intestine. Weak levels in heart and lung.
數據庫鏈接：

KEGG: mmu:17384

STRING: 10090.ENSMUSP00000034488

UniGene: Mm.14126

Mouse matrix metalloproteinase 10,MMP-10 ELISA kit

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