Mouse matrix metallopeptidase 7 (matrilysin, uterine) (MMP7) ELISA kit

Mouse matrix metallopeptidase 7 (matrilysin, uterine) (MMP7) ELISA kit

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中文名稱：

小鼠基質金屬蛋白酶7(MMP7)酶聯免疫試劑盒
貨號：

CSB-E07409m
規格：

96T/48T
價格：

￥3200/￥2500
其他：

CUSABIO Wins 2024 Bioz Rapid Star? Award in the ELISA category

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產品描述：

小鼠基質金屬蛋白酶7(MMP7)酶聯免疫試劑盒（CSB-E07409m）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織培養上清液、組織勻漿樣本中的MMP7含量。MMP7即基質金屬蛋白酶7，在細胞外基質降解、腫瘤發生發展等過程中發揮重要作用。其研究機制主要聚焦于它對基底膜和細胞外基質成分的降解，促進腫瘤細胞侵襲轉移，還參與炎癥反應、血管生成等，是腫瘤等疾病的潛在治療靶點。試劑盒檢測范圍為31.25 pg/mL-2000 pg/mL，適用于多種科研場景，如腫瘤微環境研究中MMP7的表達水平分析、炎癥性疾病模型中的蛋白酶活性評估，或組織修復機制探索實驗中細胞分泌動態監測。其寬泛的樣本兼容性支持研究人員針對不同實驗體系（如體外細胞培養分泌檢測、動物模型組織提取物分析等）開展定量研究，為探究MMP7在疾病發生發展中的調控作用提供可靠工具。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
別名：

Mmp7Matrilysin ELISA Kit; EC 3.4.24.23 ELISA Kit; Matrin ELISA Kit; Matrix metalloproteinase-7 ELISA Kit; MMP-7 ELISA Kit; Pump-1 protease ELISA Kit; Uterine metalloproteinase ELISA Kit
縮寫：

MMP7
Uniprot No.：

Q10738
種屬：

Mus musculus (Mouse)
樣本類型：

serum, plasma, cell culture supernates, tissue homogenates
檢測范圍：

31.25 pg/mL-2000 pg/mL
靈敏度：

7.81 pg/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Cancer
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of mouse MMP7 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	93
	Range %	90-95
1:2	Average %	102
	Range %	98-104
1:4	Average %	92
	Range %	88-96
1:8	Average %	99
	Range %	95-102

回收率：

The recovery of mouse MMP7 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	94	92-96
EDTA plasma (n=4)	96	92-99

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
2000	2.180	2.088	2.134	1.981
1000	1.548	1.534	1.541	1.388
500	0.882	0.899	0.891	0.738
250	0.511	0.489	0.500	0.347
125	0.338	0.331	0.335	0.182
62.5	0.256	0.248	0.252	0.099
31.25	0.202	0.211	0.207	0.054
0	0.154	0.152	0.153

數據處理：

ELISA操作視頻及標曲繪制軟件下載
貨期：

3-5 working days

Distance-dependent visual fluorescence immunoassay on CdTe quantum dot–impregnated paper through silver ion-exchange reaction L Huang,Microchimica Acta,2020

功能：

Degrades casein, gelatins of types I, III, IV, and V, and fibronectin. Activates procollagenase.; May play a role in tissue reorganization.
亞細胞定位：

Secreted, extracellular space, extracellular matrix.
蛋白家族：

Peptidase M10A family
數據庫鏈接：

STRING: 10090.ENSMUSP00000018767

UniGene: Mm.4825

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.