Mouse low density lipoprotein cholesterol (LDL-C) ELISA kit

文獻引用 (6) 說明書下載

產(chǎn)品詳情

產(chǎn)品描述：

小鼠低密度脂蛋白膽固醇(LDL-C)酶聯(lián)免疫試劑盒（CSB-EQ027860MO）為競爭法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的LDL-C含量。LDL-C（低密度脂蛋白膽固醇）是心血管疾病重要風險因素。其水平升高易在血管壁沉積形成斑塊。科研聚焦降低LDL-C水平以減少心血管事件。研究機制包括抑制合成、加速清除等，如開發(fā)他汀類、PCSK9抑制劑等藥物來干預該靶點。試劑盒檢測范圍為0.312 μg/mL-20 μg/mL，該產(chǎn)品可廣泛應用于小鼠模型中的脂質代謝機制研究、心血管疾病相關藥物開發(fā)、飲食干預實驗或基因工程動物模型的表型分析，為評估脂質代謝異常、動脈硬化病理進程及潛在治療策略提供可靠的實驗工具。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
縮寫：

LDL-C
種屬：

Mus musculus (Mouse)
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

0.312 μg/mL-20 μg/mL
靈敏度：

0.078 μg/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Cardiovascular
測定原理：

quantitative
測定方法：

Competitive

精密度：

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of mouse LDL-C in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:100	Average %	86
	Range %	82-92
1:200	Average %	94
	Range %	91-98
1:400	Average %	107
	Range %	103-114
1:800	Average %	89
	Range %	85-94

回收率：

The recovery of mouse LDL-Cspiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	88	84-93
EDTA plasma (n=4)	91	87-95

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.