Mouse cross linked N-telopeptide of type Ⅰ collagen,NTX ELISA Kit

產品詳情

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Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產品描述：

小鼠Ⅰ型膠原N末端肽(NTX)酶聯免疫試劑盒（CSB-E09242m）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的NTX含量。小鼠Ⅰ型膠原N末端肽（NTX）是Ⅰ型膠原蛋白的N端肽段，具有特征性的D-Band結構，對骨生物礦化至關重要。NTX在骨修復材料中發揮重要作用，其穩定的三螺旋結構和自組裝能力對形成具有生物礦化能力的膠原纖維至關重要。試劑盒檢測范圍為3.12 nM BCE-200 nM BCE，常用于評估骨質疏松、骨折修復及骨重塑相關機制的研究；適用于體外實驗中對骨代謝疾病模型、藥物干預效果或骨骼發育調控的科研分析，可為骨生物學、藥理學及再生醫學領域提供可靠的數據支持本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
別名：

cross linked N-telopeptide of type 1 collagen,NTX,_x000D_ cross linked N-telopeptide of type I collagen,NTX
縮寫：

NTX
種屬：

Mus musculus (Mouse)
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

3.12 nM BCE-200 nM BCE
靈敏度：

0.78 nM BCE
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Neuroscience
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of mouse NTX in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	92
	Range %	88-96
1:2	Average %	88
	Range %	82-95
1:4	Average %	99
	Range %	94-103
1:8	Average %	102
	Range %	96-108

回收率：

The recovery of mouse NTX spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	90	85-96
EDTA plasma (n=4)	98	95-101

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.