Mouse coagulation factor Ⅺ,FⅪ ELISA Kit

Mouse coagulation factor Ⅺ,FⅪ ELISA Kit

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中文名稱：

小鼠凝血因子Ⅺ(FⅪ)酶聯免疫試劑盒
貨號：

CSB-E17818m
規格：

96T/48T
價格：

￥3600/￥2500
其他：

CUSABIO Wins 2024 Bioz Rapid Star? Award in the ELISA category

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產品描述：

小鼠凝血因子Ⅺ(FⅪ)酶聯免疫試劑盒（CSB-E17818m）為競爭法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的F11含量。試劑盒檢測范圍為0.47 ng/mL-30 ng/mL，該產品可應用于凝血機制研究、血栓性疾病動物模型分析、抗凝藥物開發等科研領域，尤其適用于心血管疾病模型小鼠的血液樣本檢測，以及肝、腎等組織中FⅪ表達水平的比較研究，為探索凝血因子調控機制提供可靠工具。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
別名：

F11Coagulation factor XI ELISA kit; FXI ELISA kit; EC 3.4.21.27 ELISA kit; Plasma thromboplastin antecedent ELISA kit; PTA) [Cleaved into: Coagulation factor XIa heavy chain; Coagulation factor XIa light chain] ELISA kit
縮寫：

F
Uniprot No.：

Q91Y47
種屬：

Mus musculus (Mouse)
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

0.47 ng/mL-30 ng/mL
靈敏度：

0.12 ng/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Blood Coagulation
測定原理：

quantitative
測定方法：

Competitive

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of mouse FⅪ in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:100	Average %	95
	Range %	90-101
1:200	Average %	87
	Range %	84-90
1:400	Average %	102
	Range %	98-106
1:800	Average %	93
	Range %	89-97

回收率：

The recovery of mouse FⅪ spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	94	90-98
EDTA plasma (n=4)	98	94-103

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average
30	0.147	0.139	0.143
15	0.219	0.225	0.222
7.5	0.345	0.326	0.336
3.75	0.513	0.536	0.525
1.875	0.857	0.862	0.860
0.94	1.179	1.198	1.189
0.47	1.538	1.559	1.549
0	2.533	2.542	2.538

數據處理：

ELISA操作視頻及標曲繪制軟件下載
貨期：

3-5 working days

功能：

Factor XI triggers the middle phase of the intrinsic pathway of blood coagulation by activating factor IX.
基因功能參考文獻：
1. FXI(-/-) mice have a mild hemostatic defect after injury to the saphenous vein but not after transection of the tail or vessels in the abdominal wall. PMID: 28677246
2. FXI protects against sepsis derived from Streptococcus pneumoniae or Klebsiella pneumoniae pneumonia at least in part by enhancing the phagocytic capacity of neutrophils by a mechanism that is independent of activation via FXIIa. PMID: 28492700
3. FXI attenuates part of the allergic response to repeated administration of house dust mite in the airways by a mechanism that is independent of activation via FXII. PMID: 27913422
4. Thrombin-independent contribution of tissue factor to inflammation and cardiac hypertrophy in a mouse model of sickle cell disease. PMID: 26817955
5. FXI deprivation was shown to slow down atherogenesis in Apoe knockout mice. PMID: 26800563
6. anion-binding sites on FXIa that are required for expression of heparin's cofactor activity during protease inhibition by antithrombin are also required for expression of polyP cofactor activity during FXI activation PMID: 24118982
7. Factor XI antisense oligonucleotides safely prevent thrombus formation on acutely ruptured atherosclerotic plaques in mice. PMID: 24947525
8. Studies indicate that targeting factor XI (FXI) by knocking out the gene prevents arterial and vein thrombosis. PMID: 23929304
9. Protective roles for fibrin, tissue factor, plasminogen activator inhibitor-1, and thrombin activatable fibrinolysis inhibitor, but not factor XI, during defense against the gram-negative bacterium Yersinia enterocolitica. PMID: 21724997
10. fXI and fXII contribute to thrombus formation even when factor VIIa/tissue factor initiates thrombosis. PMID: 20634381
11. Gene targeting of tissue factor, factor X, and factor VII in mice: their involvement in embryonic development PMID: 11841337
12. FXI is essential for thrombus formation following FeCl3-induced injury of the carotid artery in the mouse. PMID: 12008966
13. FIX and FXI deficiency cause similar effects in the carotid artery injury model PMID: 15733058
14. fXI may have functions in regulation of inflammation or tissue repair distinct from its role in coagulation PMID: 15985536
15. apolipoprotein E receptor 2 (ApoER2, LRP8), a member of the LDL receptor family, is a platelet receptor for FXI. PMID: 19661487
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亞細胞定位：

Secreted.
蛋白家族：

Peptidase S1 family, Plasma kallikrein subfamily
數據庫鏈接：

KEGG: mmu:109821

STRING: 10090.ENSMUSP00000034064

UniGene: Mm.33326

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.