Mouse coagulation factor Ⅶ,FⅦ ELISA Kit

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中文名稱：

小鼠凝血因子Ⅶ(FⅦ)酶聯免疫試劑盒
貨號：

CSB-E17817m
規格：

96T/48T
價格：

￥3600/￥2500
促銷：
其他：

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產品描述：

小鼠凝血因子Ⅶ(FⅦ)酶聯免疫試劑盒（CSB-E17817m）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的F7含量。小鼠凝血因子Ⅶ（FⅦ）是凝血系統中重要的因子，其基因剔除小鼠模型在研究凝血機制和抗血栓藥物靶點方面具有重要作用。FⅦ基因剔除小鼠因缺乏FⅦ而表現出嚴重的出血傾向，為研究凝血因子功能提供了有力工具。試劑盒檢測范圍為0.45 ng/mL-30 ng/mL，該產品適用于凝血機制基礎研究、抗凝藥物開發評估以及血栓性疾病或出血性疾病小鼠模型中FⅦ蛋白水平的動態監測，為研究凝血調控網絡提供可靠工具。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
別名：

F7 ELISA Kit; Cf7 ELISA Kit; Coagulation factor VII ELISA Kit; EC 3.4.21.21 ELISA Kit; Serum prothrombin conversion accelerator) [Cleaved into: Factor VII light chain; Factor VII heavy chain] ELISA Kit
縮寫：

F7
Uniprot No.：

P70375
種屬：

Mus musculus (Mouse)
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

0.45 ng/mL-30 ng/mL
靈敏度：

0.11 ng/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Blood Coagulation
測定原理：

quantitative
測定方法：

Sandwich

精密度：

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of mouse FⅦ in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1000	Average %	92
	Range %	86-96
1:2000	Average %	97
	Range %	92-102
1:4000	Average %	93
	Range %	88-97
1:8000	Average %	89
	Range %	84-94

回收率：

The recovery of mouse FⅦ spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	92	88-95
EDTA plasma (n=4)	108	103-114

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
30	2.468	2.445	2.457	2.324
15	2.115	1.951	2.033	1.900
7.5	1.503	1.440	1.472	1.339
3.75	0.845	0.790	0.818	0.685
1.8	0.626	0.593	0.610	0.477
0.9	0.381	0.362	0.372	0.239
0.45	0.274	0.252	0.263	0.130
0	0.137	0.129	0.133

數據處理：

ELISA操作視頻及標曲繪制軟件下載
貨期：

3-5 working days

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功能：

Initiates the extrinsic pathway of blood coagulation. Serine protease that circulates in the blood in a zymogen form. Factor VII is converted to factor VIIa by factor Xa, factor XIIa, factor IXa, or thrombin by minor proteolysis. In the presence of tissue factor and calcium ions, factor VIIa then converts factor X to factor Xa by limited proteolysis. Factor VIIa will also convert factor IX to factor IXa in the presence of tissue factor and calcium.
基因功能參考文獻：
1. Leu8 is crucial for FVIIa-EPCR binding; this study characterizes its interaction in vivo in mice PMID: 28035745
2. FVIIa-antithrombin but not FVIIa is a ligand for LRP1, and LRP1 contributes to the clearance of FVIIa-antithrombin in vivo PMID: 27614059
3. FVIIa binding to EPCR leads to a barrier protective effect in vivo PMID: 24977291
4. Murine FVIIa binds poorly to murine EPCR. PMID: 22370814
5. GlycoPEGylated rFVIIa (N7-GP) has a prolonged hemostatic effect in hemophilic mice compared with rFVIIa PMID: 21366858
6. Conclude that the fVII-targeted verteporfin photodynamic therapy that we report here is a novel and effective therapeutic with improved selectivity for the treatment of breast cancer. PMID: 20504328
7. Recombinant FVIIa readily associates with the vascular endothelium and subsequently enters into extravascular spaces where it is likely to bind to tissue factor and is retained for extended time periods. PMID: 19943873
8. true circadian rhythms for FVII were found PMID: 15604416
9. Data suggest that long-term expression of murine activated factor VII is safe, but elevated levels cause premature mortality. PMID: 18398505
10. FVIIa functions in the asthmatic response to an allergen by stimulating lung eosinophilia, airway hyperresponsiveness, and mucin production. PMID: 19286924
11. Plasma elimination kinetics for factor VII are independent of its activation to factor VIIa and complex formation with plasma inhibitors. PMID: 19404533
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亞細胞定位：

Secreted.
蛋白家族：

Peptidase S1 family
組織特異性：

Plasma and liver.
數據庫鏈接：

KEGG: mmu:14068

STRING: 10090.ENSMUSP00000033820

UniGene: Mm.4827

Mouse coagulation factor Ⅶ,FⅦ ELISA Kit

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