Mouse circulating immune complex(CIC)ELISA Kit

Mouse circulating immune complex(CIC)ELISA Kit

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中文名稱：

小鼠循環(huán)免疫復(fù)合物(CIC)酶聯(lián)免疫試劑盒
貨號(hào)：

CSB-E16553m
規(guī)格：

96T/48T
價(jià)格：

￥3600/￥2500
其他：

CUSABIO Wins 2024 Bioz Rapid Star? Award in the ELISA category

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產(chǎn)品描述：

小鼠循環(huán)免疫復(fù)合物(CIC)酶聯(lián)免疫試劑盒（CSB-E16553m）為雙抗夾心法ELISA試劑盒，定量檢測(cè)血清、血漿樣本中的CIC含量。小鼠循環(huán)免疫復(fù)合物（CIC）是體內(nèi)抗原與抗體結(jié)合形成的復(fù)合物，多數(shù)情況下對(duì)機(jī)體有益，但在某些情況下，如免疫復(fù)合物沉積，可能引起組織損傷和疾病。CIC的研究涉及其形成機(jī)制、臨床意義以及與疾病如類風(fēng)濕關(guān)節(jié)炎的關(guān)系。試劑盒檢測(cè)范圍為15.6 ng/mL-1000 ng/mL，適用于小鼠自身免疫疾病模型構(gòu)建、感染性疾病研究中免疫復(fù)合物沉積機(jī)制探索，或藥物干預(yù)后免疫調(diào)節(jié)效應(yīng)的評(píng)估，也可用于免疫學(xué)、病理學(xué)等基礎(chǔ)研究中對(duì)樣本中 CIC 水平進(jìn)行檢測(cè)以探究免疫調(diào)節(jié)機(jī)制或評(píng)估疾病模型狀態(tài)。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
別名：

N/A
縮寫：

CIC
種屬：

Mus musculus (Mouse)
樣本類型：

serum, plasma
檢測(cè)范圍：

15.6 ng/mL-1000 ng/mL
靈敏度：

3.9 ng/mL
反應(yīng)時(shí)間：

1-5h
樣本體積：

50-100ul
檢測(cè)波長(zhǎng)：

450 nm
研究領(lǐng)域：

Others
測(cè)定原理：

quantitative
測(cè)定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of mouse CIC in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:20	Average %	95
	Range %	89-103
1:40	Average %	90
	Range %	81-100
1:80	Average %	91
	Range %	86-99
1:160	Average %	95
	Range %	91-104

回收率：

The recovery of mouse CIC spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	89-102
EDTA plasma (n=4)	96	90-104

標(biāo)準(zhǔn)曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
1000	2.107	2.053	2.080	1.923
500	1.593	1.575	1.584	1.427
250	1.097	1.023	1.060	0.903
125	0.635	0.611	0.623	0.466
62.5	0.449	0.426	0.438	0.281
31.2	0.302	0.283	0.293	0.136
15.6	0.226	0.217	0.222	0.065
0	0.152	0.162	0.157

數(shù)據(jù)處理：

ELISA操作視頻及標(biāo)曲繪制軟件下載
貨期：

3-5 working days

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.