Mouse choline acetyltransterase(ChAT)ELISA Kit

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產(chǎn)品詳情

產(chǎn)品描述：

小鼠膽堿乙酰轉(zhuǎn)移酶（ChAT）酶聯(lián)免疫試劑盒（CSB-E17524m）為雙抗夾心法ELISA試劑盒，定量檢測(cè)血清、血漿、組織勻漿、組織培養(yǎng)上清液樣本中的CHAT含量。試劑盒檢測(cè)范圍為15.6 pg/mL-1000 pg/mL，在神經(jīng)退行性疾病模型研究、神經(jīng)發(fā)育機(jī)制探索及藥物干預(yù)效果評(píng)估中具有重要科研價(jià)值。為神經(jīng)科學(xué)、藥理學(xué)及基礎(chǔ)醫(yī)學(xué)研究提供穩(wěn)定高效的檢測(cè)工具。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說(shuō)明書。
別名：

ChatCholine O-acetyltransferase ELISA kit; CHOACTase ELISA kit; ChAT ELISA kit; Choline acetylase ELISA kit; EC 2.3.1.6 ELISA kit
縮寫：

CHAT
Uniprot No.：

Q03059
種屬：

Mus musculus (Mouse)
樣本類型：

serum, plasma, tissue homogenates, cell culture supernates
檢測(cè)范圍：

15.6 pg/mL-1000 pg/mL
靈敏度：

3.9 pg/mL
反應(yīng)時(shí)間：

1-5h
樣本體積：

50-100ul
檢測(cè)波長(zhǎng)：

450 nm
研究領(lǐng)域：

Neuroscience
測(cè)定原理：

quantitative
測(cè)定方法：

Sandwich

精密度：

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of mouse ChAT in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:100	Average %	92
	Range %	88-96
1:200	Average %	87
	Range %	82-91
1:400	Average %	103
	Range %	99-108
1:800	Average %	95
	Range %	90-100

回收率：

The recovery of mouse ChAT spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	96	91-100
EDTA plasma (n=4)	92	88-96

標(biāo)準(zhǔn)曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

功能：

Catalyzes the reversible synthesis of acetylcholine (ACh) from acetyl CoA and choline at cholinergic synapses.
蛋白家族：

Carnitine/choline acetyltransferase family
數(shù)據(jù)庫(kù)鏈接：

STRING: 10090.ENSMUSP00000070865

UniGene: Mm.442817