Mouse calcitonin gene related peptide (CGRP) ELISA kit

Mouse calcitonin gene related peptide (CGRP) ELISA kit

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中文名稱：

小鼠降鈣素基因相關(guān)肽(CGRP)酶聯(lián)免疫試劑盒
貨號(hào)：

CSB-EQ027706MO
規(guī)格：

96T/48T
價(jià)格：

￥3600/￥2500
其他：

CUSABIO Wins 2024 Bioz Rapid Star? Award in the ELISA category

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產(chǎn)品描述：

小鼠降鈣素基因相關(guān)肽(CGRP)酶聯(lián)免疫試劑盒（CSB-EQ027706MO）檢測(cè)血清、血漿、組織勻漿樣本中的CGRP含量。CGRP（降鈣素基因相關(guān)肽）是一種神經(jīng)肽，主要在感覺神經(jīng)纖維表達(dá)，如C和Aδ感覺纖維，廣泛分布于全身，尤其在血管周圍。CGRP在偏頭痛的發(fā)病機(jī)制中起關(guān)鍵作用，通過促進(jìn)神經(jīng)源性炎癥和顱腦血管擴(kuò)張引發(fā)偏頭痛。近年來，針對(duì)CGRP及其受體的藥物研發(fā)成為偏頭痛治療的熱點(diǎn)。試劑盒檢測(cè)范圍為15.6 pg/mL-1000 pg/mL，支持科研人員探究CGRP在神經(jīng)科學(xué)、藥理學(xué)或疾病模型中的動(dòng)態(tài)變化，例如分析疼痛模型中神經(jīng)遞質(zhì)釋放規(guī)律、評(píng)估藥物對(duì)CGRP通路的調(diào)控作用，或研究心血管疾病與神經(jīng)肽關(guān)聯(lián)機(jī)制。本試劑盒適用于多種生物樣本類型，為神經(jīng)生物學(xué)、免疫學(xué)及代謝性疾病等基礎(chǔ)研究領(lǐng)域提供標(biāo)準(zhǔn)化檢測(cè)工具。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
別名：

Beta type CGRP、Calcitonin gene-related peptide II、CGRPII、Calcitonin gene-related peptide 2、CALCB
縮寫：

CGRP
Uniprot No.：

Q99MP3
種屬：

Mus musculus (Mouse)
樣本類型：

serum, plasma, tissue homogenates
檢測(cè)范圍：

15.6 pg/mL-1000 pg/mL
靈敏度：

3.9 pg/mL
反應(yīng)時(shí)間：

1-5h
樣本體積：

50-100ul
檢測(cè)波長(zhǎng)：

450 nm
研究領(lǐng)域：

Signal Transduction
測(cè)定原理：

quantitative
測(cè)定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of mouse CGRP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	94
Range %	86-98
1:2	Average %	92
Range %	88-97
1:4	Average %	90
Range %	85-96
1:8	Average %	94
Range %	88-99

回收率：

The recovery of mouse CGRP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	93	89-97
EDTA plasma (n=4)	96	91-99

標(biāo)準(zhǔn)曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
1000	2.342	2.321	2.332	2.178
500	1.772	1.760	1.766	1.612
250	1.250	1.241	1.246	1.092
125	0.799	0.789	0.794	0.640
62.5	0.499	0.487	0.493	0.339
31.25	0.364	0.341	0.353	0.199
15.6	0.253	0.244	0.249	0.095
0	0.156	0.152	0.154

數(shù)據(jù)處理：

ELISA操作視頻及標(biāo)曲繪制軟件下載
貨期：

3-5 working days

Sequential SDF-1/CGRP-releasing smart composite hydrogel promotes osteoporotic fracture healing by targeting sensory nerve-regulated bone remodeling Y Wang, Z Pan, Q Wang, Y Shu, Z Tan, Y Chen, J He,Materials Today Bio,2025
Nociceptor neurons promote PDAC progression and cancer pain by interaction with cancer-associated fibroblasts and suppression of natural killer cells K Wang, B Ni, Y Xie, Z Li, L Yuan, C Meng, T Zhao, S Gao, C Huang, H Wang, Y Ma, T Zhou,Cell Research,2025
Gender-different effect of Src family kinases antagonism on photophobia and trigeminal ganglion activity Z Huang, J Yao, L Nie, X Nie, X Xiong, S K?ks,The Journal of Headache and Pain,2024
Transcript-dependent effects of the CALCA gene on the progression of post-traumatic osteoarthritis in mice S Jiang,Communications biology,2024
Immune tolerance induced in the anterior chamber ameliorates corneal transplant rejection J Sun,Clinical immunology,2023
Src Family Kinases Facilitate the Crosstalk between CGRP and Cytokines in Sensitizing Trigeminal Ganglion via Transmitting CGRP Receptor/PKA Pathway L Nie,Cells,2022
Adipose mTORC2 is essential for sensory innervation in white adipose tissue and whole-body energy homeostasis IC Frei,Molecular metabolism,2022
Interleukin-17A is involved in mechanical hyperalgesia but not in the severity of murine antigen-induced arthritis Matthias Ebbinghaus.et al,Sci Rep,2017
Chemical profiles and pharmacological activities of Chang-Kang-Fang, a multi-herb Chinese medicinal formula, for treating irritable bowel syndrome Qian Mao.et al,Journal of Ethnopharmacology,2017

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平均分:

5.0分 - 2 個(gè)評(píng)價(jià)

樣品類型：組織勻漿液小鼠神經(jīng)節(jié)組織

樣品信息：小鼠

稀釋比：沒有稀釋

產(chǎn)品評(píng)價(jià)： ELISA試劑盒在檢測(cè)CGRP時(shí)展現(xiàn)了較高的靈敏度和特異性。在小鼠drg樣本中，重復(fù)性良好。試劑盒的操作簡(jiǎn)便，且廠家提供了詳細(xì)的實(shí)驗(yàn)步驟和在線技術(shù)支持，幫助解決了實(shí)驗(yàn)中的問題。ELISA試劑盒的檢測(cè)范圍（最低和最高濃度）也適應(yīng)實(shí)驗(yàn)的需求。具備高靈敏度，能夠在低濃度下準(zhǔn)確檢測(cè)CGRP。

By 閆老師

樣品類型：組織勻漿液小鼠肺組織勻漿液

樣品信息：小鼠

稀釋比：1:50

產(chǎn)品評(píng)價(jià)：我使用CSB-EQ027706MO檢測(cè)小鼠肺組織勻漿液樣本，標(biāo)準(zhǔn)曲線的 OD 值在 0.176 - 1.749 之間呈現(xiàn)良好線性關(guān)系，樣本 OD 值大致在 0.2 - 1.7 區(qū)間。試劑盒的重復(fù)性較好，多次平行實(shí)驗(yàn)結(jié)果波動(dòng)比較小。從操作體驗(yàn)來說，試劑預(yù)分裝設(shè)計(jì)十分貼心，減少了繁瑣的準(zhǔn)備步驟，節(jié)省了時(shí)間。而且靈敏度較高，能精準(zhǔn)檢測(cè)到CGRP，為科研數(shù)據(jù)提供了可靠支撐，是一款非常值得信賴的 ELISA 試劑盒。

By 王老師

功能：

CGRP induces vasodilation. It dilates a variety of vessels including the coronary, cerebral and systemic vasculature. Its abundance in the CNS also points toward a neurotransmitter or neuromodulator role.
亞細(xì)胞定位：

Secreted.
蛋白家族：

Calcitonin family
組織特異性：

Detected in nerve cells of cerebrum, hippocampus and pons/midbrain in newborns, and only in nerve cells of pons/midbrain in adult.
數(shù)據(jù)庫(kù)鏈接：

STRING: 10090.ENSMUSP00000032902

UniGene: Mm.273838

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.