Mouse anti-Smith antibody ELISA Kit

產(chǎn)品詳情

文獻(xiàn)引用 (6) 說(shuō)明書下載

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產(chǎn)品描述：

小鼠抗Smith抗體(Sm)酶聯(lián)免疫試劑盒（CSB-E15976m）為雙抗夾心法ELISA試劑盒，定量檢測(cè)血清、血漿樣本中的Sm Ab含量。Sm Ab即抗史密斯抗體，是系統(tǒng)性紅斑狼瘡（SLE）的血清標(biāo)志性抗體。其背景在于SLE是一種自身免疫病。研究機(jī)制方面，它能識(shí)別細(xì)胞核中的特定蛋白，可作為SLE診斷的重要依據(jù)，在自身免疫反應(yīng)和疾病發(fā)生發(fā)展機(jī)制研究中有重要意義。試劑盒檢測(cè)范圍為0.625 ng/mL-40 ng/mL，適用于科研領(lǐng)域中對(duì)系統(tǒng)性紅斑狼瘡（SLE）相關(guān)模型的抗體水平分析；可廣泛應(yīng)用于小鼠疾病模型中自身抗體動(dòng)態(tài)監(jiān)測(cè)、藥物療效評(píng)估或免疫病理學(xué)研究；為探索SLE發(fā)病機(jī)理及潛在治療靶點(diǎn)提供可靠工具本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說(shuō)明書。
縮寫：

Sm Ab
種屬：

Mus musculus (Mouse)
樣本類型：

serum, plasma
檢測(cè)范圍：

0.625 ng/mL-40 ng/mL
靈敏度：

0.156 ng/mL
反應(yīng)時(shí)間：

1-5h
樣本體積：

50-100ul
檢測(cè)波長(zhǎng)：

450 nm
研究領(lǐng)域：

Immunology
測(cè)定原理：

quantitative
測(cè)定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of mouse anti-smith antibody in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	88
	Range %	81-95
1:2	Average %	95
	Range %	90-100
1:4	Average %	93
	Range %	92-101
1:8	Average %	93
	Range %	82-98

回收率：

The recovery of mouse anti-smith antibody spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	92	85-99
EDTA plasma (n=4)	95	90-100

標(biāo)準(zhǔn)曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.