Mouse amyloid beta peptide 1-40,Aβ1-40 ELISA Kit

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中文名稱：

小鼠β淀粉樣蛋白1-40(Aβ1-40)酶聯(lián)免疫試劑盒
貨號(hào)：

CSB-E08300m
規(guī)格：

96T/48T
價(jià)格：

￥3800/￥2500
促銷：
其他：

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Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產(chǎn)品描述：

小鼠β淀粉樣蛋白1-40(Aβ1-40)酶聯(lián)免疫試劑盒（CSB-E08300m）為雙抗夾心法ELISA試劑盒，定量檢測(cè)血清、血漿、組織勻漿樣本中的Aβ1-40含量。Aβ1-40是一種淀粉樣蛋白，是阿爾茨海默病（AD）病理過(guò)程中的關(guān)鍵分子。它是淀粉樣蛋白前體蛋白（APP）的代謝產(chǎn)物，通過(guò)β-折疊結(jié)構(gòu)聚集形成老年斑，對(duì)神經(jīng)細(xì)胞產(chǎn)生毒性，導(dǎo)致神經(jīng)元損傷和認(rèn)知功能下降。Aβ1-40的研究有助于揭示AD的發(fā)病機(jī)制，并為開(kāi)發(fā)新的治療策略提供靶點(diǎn)。試劑盒檢測(cè)范圍為31.25 pg/mL-2000 pg/mL，主要應(yīng)用于神經(jīng)科學(xué)領(lǐng)域的基礎(chǔ)研究，包括阿爾茨海默病小鼠模型建立、藥物干預(yù)后Aβ代謝通路評(píng)估、神經(jīng)毒性作用機(jī)制探索等方向，為病理機(jī)制解析和潛在治療策略開(kāi)發(fā)提供可靠數(shù)據(jù)支持。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見(jiàn)產(chǎn)品說(shuō)明書。
別名：

amyloid beta peptide 1-40,Aβ1-40,amyloid β peptide 1-40
縮寫：

Aβ1-40
種屬：

Mus musculus (Mouse)
樣本類型：

serum, plasma, tissue homogenates
檢測(cè)范圍：

31.25 pg/mL-2000 pg/mL
靈敏度：

7.8 pg/mL
反應(yīng)時(shí)間：

1-5h
樣本體積：

50-100ul
檢測(cè)波長(zhǎng)：

450 nm
研究領(lǐng)域：

Neuroscience
測(cè)定原理：

quantitative
測(cè)定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of mouse Aβ1-40 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	89
	Range %	80-97
1:2	Average %	95
	Range %	90-105
1:4	Average %	95
	Range %	83-100
1:8	Average %	93
	Range %	83-98

回收率：

The recovery of mouse Aβ1-40 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	97	93-106
EDTA plasma (n=4)	89	86-92

標(biāo)準(zhǔn)曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
2000	2.637	2.739	2.688	2.512
1000	2.115	2.092	2.104	1.928
500	1.318	1.287	1.303	1.127
250	0.824	0.810	0.817	0.641
125	0.491	0.504	0.498	0.322
62.5	0.322	0.315	0.319	0.143
31.25	0.265	0.273	0.269	0.093
0	0.172	0.180	0.176