Mouse adrenocorticotropic hormone (ACTH) ELISA kit

Mouse adrenocorticotropic hormone (ACTH) ELISA kit

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中文名稱：

小鼠促腎上腺皮質(zhì)激素(ACTH)酶聯(lián)免疫試劑盒
貨號(hào)：

CSB-E06874m
規(guī)格：

96T/48T
價(jià)格：

￥3800/￥2500
其他：

CUSABIO Wins 2024 Bioz Rapid Star? Award in the ELISA category

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產(chǎn)品描述：

小鼠促腎上腺皮質(zhì)激素(ACTH)酶聯(lián)免疫試劑盒（CSB-E06874m）為雙抗夾心法ELISA試劑盒，定量檢測(cè)血清、血漿樣本中的ACTH含量。ACTH即促腎上腺皮質(zhì)激素，由腺垂體分泌。其背景在于它能促進(jìn)腎上腺皮質(zhì)的組織增生以及皮質(zhì)激素的生成和分泌。研究機(jī)制方面，它作用于腎上腺皮質(zhì)細(xì)胞上的受體，激活相關(guān)信號(hào)通路，調(diào)控皮質(zhì)激素合成關(guān)鍵酶的表達(dá)，進(jìn)而調(diào)節(jié)機(jī)體應(yīng)激等生理過程。試劑盒檢測(cè)范圍為18.75 pg/mL-1200 pg/mL，該產(chǎn)品適用于實(shí)驗(yàn)動(dòng)物模型中ACTH動(dòng)態(tài)變化的分析，例如應(yīng)激反應(yīng)評(píng)估、腎上腺功能研究、內(nèi)分泌調(diào)控機(jī)制探索以及藥物或環(huán)境因素對(duì)激素分泌影響的科研實(shí)驗(yàn)，為神經(jīng)內(nèi)分泌領(lǐng)域的定量研究提供可靠工具。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
縮寫：

ACTH
種屬：

Mus musculus (Mouse)
樣本類型：

serum, plasma
檢測(cè)范圍：

18.75 pg/mL-1200 pg/mL
靈敏度：

4.68 pg/mL
反應(yīng)時(shí)間：

1-5h
樣本體積：

50-100ul
檢測(cè)波長：

450 nm
研究領(lǐng)域：

Metabolism
測(cè)定原理：

quantitative
測(cè)定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of mouse ACTH in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	92
	Range %	80-105
1:2	Average %	96
	Range %	85-104
1:4	Average %	98
	Range %	92-104
1:8	Average %	96
	Range %	82-110

回收率：

The recovery of mouse ACTH spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	84-102
EDTA plasma (n=4)	98	90-105

標(biāo)準(zhǔn)曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
1200	3.134	3.126	3.130	2.988
600	2.537	2.532	2.535	2.393
300	1.754	1.765	1.760	1.618
150	1.053	1.050	1.052	0.910
75	0.651	0.647	0.649	0.507
37.5	0.443	0.435	0.439	0.297
18.75	0.273	0.265	0.269	0.127
0	0.140	0.144	0.142

數(shù)據(jù)處理：

ELISA操作視頻及標(biāo)曲繪制軟件下載
貨期：

3-5 working days

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Single-cell transcriptomic analysis reveals rich pituitary–Immune interactions under systemic inflammation T Yan,PLoS biology,2023
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Magnolol abrogates chronic mild stress-induced depressive-like behaviors by inhibiting neuroinflammation and oxidative stress in the prefrontal cortex of mice Cheng J.et al,Int Immunopharmacol.,2018
Melatonin alleviates weanling stress in mice: involvement of intestinal microbiota Ren W.et al,J Pineal Res.,2017

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平均分:

5.0分 - 2 個(gè)評(píng)價(jià)

樣品類型：血清

樣品信息：小鼠

稀釋比：其他 1:4

產(chǎn)品評(píng)價(jià)：使用CSB-E06874m試劑盒檢測(cè)小鼠血清ACTH水平，稀釋4倍后正常與模型組及各給藥組大部分均在標(biāo)曲范圍內(nèi)，但如果樣品充足可考慮1:1稀釋或使用原液，換算稀釋倍數(shù)后原液仍在標(biāo)曲范圍內(nèi)。全部實(shí)驗(yàn)在6小時(shí)內(nèi)完成，檢測(cè)結(jié)果較穩(wěn)定，標(biāo)曲良好.

By 蘭老師

樣品類型：血清

樣品信息：小鼠

稀釋比：其他 1:5

產(chǎn)品評(píng)價(jià)：小鼠血清稀釋5倍后依然在標(biāo)曲檢測(cè)范圍內(nèi)，節(jié)省樣本，對(duì)珍貴樣本極其友好；而且復(fù)孔間差異很小，兩個(gè)復(fù)孔吸光值幾乎一致，質(zhì)量很好的試劑盒。ACTH 小鼠血清稀釋5倍后樣本OD值在0.304-0.713之間。

By 羅老師

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.