Mouse Vitamin K-dependent protein S(PROS1) ELISA kit

Mouse Vitamin K-dependent protein S(PROS1) ELISA kit

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中文名稱：

小鼠維生素K依賴性蛋白S(PROS1)酶聯免疫試劑盒
貨號：

CSB-EL018754MO
規格：

96T/48T
價格：

￥3600/￥2500
促銷：
其他：

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產品描述：

小鼠維生素K依賴性蛋白S(PROS1)酶聯免疫試劑盒（CSB-EL018754MO）為競爭法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的PROS1含量。PROS1編碼的蛋白S是一種維生素K依賴的血漿糖蛋白，作為天然抗凝劑發揮作用。研究機制方面，它可增強活化蛋白C的活性以滅活凝血因子，還能以不依賴活化蛋白C的方式抑制凝血酶原酶復合物的形成，進而調控凝血過程。試劑盒檢測范圍為6.25 ng/mL-400 ng/mL，適用于科研場景下的小鼠模型研究，例如探究凝血功能障礙機制、血栓性疾病動物模型的蛋白表達動態變化，或評估肝腎功能異常對PROS1合成的影響。其寬泛的線性范圍與多類型樣本兼容性（包括組織勻漿）可為心血管疾病、免疫調節及器官特異性研究提供可靠的數據支持本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
別名：

Pros1 ELISA Kit; ProsVitamin K-dependent protein S ELISA Kit
縮寫：

PROS1
Uniprot No.：

Q08761
種屬：

Mus musculus (Mouse)
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

6.25 ng/mL-400 ng/mL
靈敏度：

1.56 ng/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Cardiovascular
測定原理：

quantitative
測定方法：

Competitive

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human APOC4 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:200	Average %	92
Range %	88-96
1:400	Average %	87
Range %	84-90
1:800	Average %	95
Range %	91-99
1:1600	Average %	97
Range %	92-102

回收率：

The recovery of human APOC4 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	94	90-98
EDTA plasma (n=4)	98	94-103

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average
400	0.080	0.083	0.082
200	0.117	0.119	0.118
100	0.177	0.172	0.175
50	0.276	0.296	0.286
25	0.504	0.490	0.497
12.5	0.923	0.931	0.927
6.25	1.461	1.496	1.479
0	2.539	2.587	2.563

數據處理：

ELISA操作視頻及標曲繪制軟件下載
貨期：

3-5 working days

功能：

Anticoagulant plasma protein; it is a cofactor to activated protein C in the degradation of coagulation factors Va and VIIIa. It helps to prevent coagulation and stimulating fibrinolysis.
基因功能參考文獻：
1. An inhibitory role for PROS1 on BM-derived LCs. Collectively. PMID: 29871951
2. This study identifies a duple role for PROS1 in stem-cell quiescence and as a pro-neurogenic factor, and highlights a unique segregation of increased stem cell proliferation from enhanced neuronal differentiation. PMID: 27753164
3. Mice overexpressing protein S showed significant improvements in blood glucose level, glucose tolerance, insulin sensitivity, and insulin secretion compared with wild-type counterparts. diabetic protein S transgenic mice developed significantly less severe diabetic glomerulosclerosis than controls. PMID: 27207541
4. results support a causal relationship between the PS-K196E mutation and venous thrombosis PMID: 26251307
5. By revealing that neural stem-like cells act within the SVZ neurogenic niche as phagocytes and that the ProS/MerTK path represents an endogenous regulatory mechanism for SVZ cell phagocytic activity PMID: 25308179
6. Data indicate that activated T cells express Pros1. PMID: 23850380
7. Results demonstrate that Protein S is a Mer ligand, and is active in Mer-driven phagocytosis in the retina. PMID: 23259948
8. A self-regulatory mechanism of Toll-like receptor signalling through the suppression of Gas6 and ProS expression is described. PMID: 22043818
9. Protein S controls hypoxic/ischemic blood-brain barrier disruption through the TAM receptor Tyro3 and sphingosine 1-phosphate receptor1. PMID: 20348395
10. Data show that the spectrum of bioactivities of recombinant murine protein S with mouse plasma and smooth muscle cells is similar to that of human protein S. PMID: 19815836
11. results demonstrate that ProS is a pleiotropic anticoagulant with activated Protein C-independent activities and highlight new roles for ProS in vascular development and homeostasis PMID: 19729839
12. Pregnancy causes a decrease in APC resistance in mice, which can be explained by the elevation of protein S levels and increased TFPI activity in plasma. PMID: 19036061
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亞細胞定位：

Secreted.
組織特異性：

Plasma.
數據庫鏈接：

KEGG: mmu:19128

STRING: 10090.ENSMUSP00000023629

UniGene: Mm.127156

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.