Mouse Undercarboxylated Osteocalcin (ucOC) ELISA Kit

產(chǎn)品詳情

文獻(xiàn)引用 (1) 說明書下載

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產(chǎn)品描述：

小鼠羧化不全骨鈣素(ucOC)酶聯(lián)免疫試劑盒（CSB-E17681m）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織培養(yǎng)上清液、組織勻漿樣本中的ucOC含量。試劑盒檢測范圍為0.156 ng/mL-10 ng/mL，為骨生物學(xué)研究提供可靠工具，特別適用于骨質(zhì)疏松動物模型構(gòu)建、骨代謝調(diào)控機(jī)制研究、藥物干預(yù)效果評價等科研領(lǐng)域，其優(yōu)化的檢測體系能有效支持血清等復(fù)雜樣本中微量ucOC的穩(wěn)定檢出，為探索骨骼系統(tǒng)疾病發(fā)生發(fā)展機(jī)制提供數(shù)據(jù)支撐。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
縮寫：

ucOC
種屬：

Mus musculus (Mouse)
樣本類型：

serum, plasma, cell culture supernates, tissue homogenates
檢測范圍：

0.156 ng/mL-10 ng/mL
靈敏度：

0.039 ng/mL
反應(yīng)時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領(lǐng)域：

Others
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of mouse ucOC in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:5	Average %	85
	Range %	80-90
1:10	Average %	92
	Range %	86-96
1:20	Average %	94
	Range %	87-100
1:40	Average %	97
	Range %	92-101

回收率：

The recovery of mouse ucOC spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	93	87-98
EDTA plasma (n=4)	90	87-94

標(biāo)準(zhǔn)曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.