Mouse Thromboxane B2,TXB2 ELISA Kit

產品詳情

文獻引用 (3) 說明書下載

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產品描述：

小鼠血栓素B2(TXB2)酶聯免疫試劑盒（CSB-E08048m）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的TXB2含量。TXB2 即血栓素 B2，是血栓素 A2 的穩定水解產物。它在體內參與血小板聚集和血管收縮過程。研究機制方面，常通過檢測其水平反映體內血栓素 A2 活性，以評估血小板功能、血管內皮損傷及血栓形成傾向，在心血管疾病等研究中受關注。試劑盒檢測范圍為2.35 pg/mL-150 pg/mL，其水平變化常被用于評估血栓形成、心血管疾病及炎癥相關機制的研究；適用于心血管疾病模型、炎癥機制研究、抗血小板藥物篩選等科研領域，可為探索病理生理過程中TXB2的動態變化及其分子調控網絡提供可靠工具本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
縮寫：

TXB2
種屬：

Mus musculus (Mouse)
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

2.35 pg/mL-150 pg/mL
靈敏度：

0.58 pg/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Signal Transduction
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of mouse TXB2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	99
	Range %	95-104
1:2	Average %	89
	Range %	84-94
1:4	Average %	90
	Range %	85-96
1:8	Average %	98
	Range %	93-103

回收率：

The recovery of mouse TXB2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	97	94-102
EDTA plasma (n=4)	91	86-97

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.