Mouse Pulmonary Surfactant-associated protein B (SP-B) ELISA kit
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中文名稱(chēng)：小鼠肺表面活性物質(zhì)相關(guān)蛋白B(SP-B)酶聯(lián)免疫試劑盒
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貨號(hào)：CSB-E12638m
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規(guī)格：96T/48T
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價(jià)格：￥3200/￥2500
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其他：
產(chǎn)品詳情
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產(chǎn)品描述：小鼠肺表面活性物質(zhì)相關(guān)蛋白B(SP-B)酶聯(lián)免疫試劑盒（CSB-E12638m）為雙抗夾心法ELISA試劑盒，定量檢測(cè)血清、血漿、組織勻漿樣本中的SFTPB含量。小鼠肺表面活性物質(zhì)相關(guān)蛋白B（SP-B）是肺泡表面活性物質(zhì)的重要組成部分，對(duì)降低肺泡表面張力、維持呼吸功能至關(guān)重要。研究顯示，SP-B的表達(dá)與肺損傷、肺部疾病的發(fā)生發(fā)展密切相關(guān)，其基因多態(tài)性可能影響肺部疾病的易感性和臨床預(yù)后。通過(guò)構(gòu)建SP-B轉(zhuǎn)基因小鼠模型，有助于深入探究SP-B在肺部疾病中的作用機(jī)制。試劑盒檢測(cè)范圍為0.45 ng/mL-30 ng/mL，本產(chǎn)品適用于基礎(chǔ)科研中探究肺部生理病理機(jī)制，例如構(gòu)建急性肺損傷、肺纖維化或新生兒呼吸窘迫綜合征等動(dòng)物模型時(shí)，可通過(guò)檢測(cè)SP - B的動(dòng)態(tài)變化評(píng)估肺功能狀態(tài)。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見(jiàn)產(chǎn)品說(shuō)明書(shū)。
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別名：Sftpb ELISA Kit; Sftp3 ELISA Kit; Pulmonary surfactant-associated protein B ELISA Kit; SP-B ELISA Kit; Pulmonary surfactant-associated proteolipid SPL(Phe) ELISA Kit
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縮寫(xiě)：SFTPB
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Uniprot No.：
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種屬：Mus musculus (Mouse)
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樣本類(lèi)型：serum, plasma, tissue homogenates
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檢測(cè)范圍：0.45 ng/mL-30 ng/mL
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靈敏度：0.11 ng/mL
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反應(yīng)時(shí)間：1-5h
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樣本體積：50-100ul
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檢測(cè)波長(zhǎng)：450 nm
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研究領(lǐng)域：Signal Transduction
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測(cè)定原理：quantitative
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測(cè)定方法：Sandwich
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精密度：
Intra-assay Precision (Precision within an assay): CV%<8% Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV%<10% Three samples of known concentration were tested in twenty assays to assess. -
線性度：
To assess the linearity of the assay, samples were spiked with high concentrations of mouse SP-B in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. Sample Serum(n=4) 1:1 Average % 92 Range % 86-97 1:2 Average % 97 Range % 92-102 1:4 Average % 88 Range % 85-93 1:8 Average % 89 Range % 83-94 -
回收率：
The recovery of mouse SP-B spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. Sample Type Average % Recovery Range Serum (n=5) 91 87-96 EDTA plasma (n=4) 102 96-109 -
標(biāo)準(zhǔn)曲線：
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. 
ng/ml OD1 OD2 Average Corrected 30 2.176 2.206 2.191 2.031 15 1.511 1.544 1.528 1.368 7.5 0.897 0.970 0.934 0.774 3.75 0.612 0.618 0.615 0.455 1.8 0.362 0.392 0.377 0.217 0.9 0.279 0.267 0.273 0.113 0.45 0.225 0.220 0.223 0.063 0 0.156 0.163 0.160 -
數(shù)據(jù)處理：
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貨期：3-5 working days
引用文獻(xiàn)
- GCN5L1 regulates pulmonary surfactant production by modulating lamellar body biogenesis and trafficking in mouse alveolar epithelial cells W Xu,Cellular & molecular biology letters,2023
- Identification of vitamin D sensitive pathways during lung development Chen L.et al,Respiratory research,2016
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靶點(diǎn)詳情
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功能：Pulmonary surfactant-associated proteins promote alveolar stability by lowering the surface tension at the air-liquid interface in the peripheral air spaces. SP-B increases the collapse pressure of palmitic acid to nearly 70 millinewtons per meter.
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基因功能參考文獻(xiàn)：
- Surfactant protein B suppresses lung cancer progression by inhibiting secretory phospholipase A2 activity and arachidonic acid production. PMID: 28743125
- These data provided a plausible mechanism linking maternal vitamin D deficiency with altered postnatal lung function. PMID: 27121020
- Surfactant protein B had decreased levels in influenza A virus PR8-infected cells, which was confirmed by immunoblotting and immunofluorescence assays. PMID: 25965799
- macrophages participate in the repression of SFTPB expression by LPS, and that macrophage-released cytokines (including TNF) regulate the transcription factor CEBPB PMID: 23590297
- ErbB4 regulates the expression of surfactant protein B in fetal lung maturation, helping to prepare for the fetal-neonatal transition. PMID: 21317380
- The stimulatory effects of estrogen on Sftpb are under transcriptional control of ErbB4. PMID: 21777626
- SP-B contributes to innate host defense of the lung by supplementing the nonoxidant antimicrobial defenses of alveolar macrophages PMID: 20007532
- DNA methylation and chromatin modifications cooperate with Nkx2-1 to regulate Sftpb gene cell specific expression. PMID: 19906647
- formation of the intersubunit disulfide bridge is not critical for SP-B function PMID: 12133857
- The late asthmatic response is linked with increased surface tension and reduced surfactant protein B in mice. PMID: 12225952
- This protein inhibits endotoxin-induced lung inflammation. PMID: 12594064
- Reduction of alveolar SP-B content causes surfactant dysfunction and respiratory failure, indicating that SP-B is required for postnatal lung function. PMID: 12639841
- SP-B expression is regulated by PPARgamma in the lung PMID: 12829715
- napsin, cathepsin H, and at least one other enzyme are involved in maturation of the biologically active SP-B peptide PMID: 14766755
- Results indicate that hypoxia-induced mitogenic factor induces surfactant protein B and C production in mouse lungs and cell lines via activation of phosphatidylinositol 3-kinase/Akt and ERK1/2 mitogen-activated protein kinases. PMID: 16166744
- absence of HO-1 in the lung parenchyma, not in bone marrow-derived inflammatory cells, was responsible for enhanced SP-B downregulation and severe physiologic lung dysfunction PMID: 16309574
- Via promoter mutation analysis, adjacent TTF-1 binding sites within the proximal promoter region of SP-C were found to be essential for TTF-1/TAP26-enhanced SP-C promoter activity. PMID: 16630564
- SP-B controls surface tension by forming a surfactant phospholipid film that regulates shape and nonspecific phagocytic activity of alveolar macrophages on the alveolar surface. PMID: 16632521
- ABCA3 is necessary for lamellar body biogenesis, surfactant protein-B processing, and lung development late in gestation. PMID: 17540762
- Three novel Sp1 binding motifs were identified in the mouse SP-B promoter. PMID: 19096034
- Results support the idea that maintenance of surfactant-associated protein B expression is critical to survival during acute lung injury. PMID: 19131640
- The surface tension-lowering properties of Sftpb play an important role in the regulation of pulmonary vascular perfusion. PMID: 19202005
- Maintaining Sftpb is critical to survival during acute lung injury PMID: 19131640
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亞細(xì)胞定位：Secreted, extracellular space, surface film.
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