Mouse Protein-arginine deiminase type-4(PADI4) ELISA kit

Mouse Protein-arginine deiminase type-4(PADI4) ELISA kit

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中文名稱：

小鼠精氨酸脫亞胺酶4(PADI4)酶聯免疫試劑盒
貨號：

CSB-EL017379MO
規格：

96T/48T
價格：

￥3600/￥2500
其他：

CUSABIO Wins 2024 Bioz Rapid Star? Award in the ELISA category

產品詳情
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引用文獻
靶點詳情

產品描述：

小鼠精氨酸脫亞胺酶4(PADI4)酶聯免疫試劑盒（CSB-EL017379MO）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿、細胞裂解物樣本中的PADI4含量。PADI4 是一種頗受關注的靶點。它在多種生理和病理過程中發揮作用。研究發現其參與蛋白質瓜氨酸化過程，與自身免疫性疾病、腫瘤的發生發展等機制相關。對 PADI4 的深入研究有助于揭示疾病發病機理，為相關疾病治療提供新方向。試劑盒檢測范圍為4.7 pg/mL-300 pg/mL，適用于體外實驗，能夠支持多種研究場景，如探索炎癥性疾病模型中PADI4的表達動態、評估基因編輯或藥物干預后酶活性變化，或分析腫瘤微環境中蛋白質翻譯后修飾機制。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
別名：

Padi4 ELISA Kit; Pad4 ELISA Kit; Pdi4 ELISA Kit; Protein-arginine deiminase type-4 ELISA Kit; EC 3.5.3.15 ELISA Kit; Peptidylarginine deiminase IV ELISA Kit; Protein-arginine deiminase type IV ELISA Kit
縮寫：

PADI4
Uniprot No.：

Q9Z183
種屬：

Mus musculus (Mouse)
樣本類型：

serum, plasma, tissue homogenates, cell lysates
檢測范圍：

4.7 pg/mL-300 pg/mL
靈敏度：

1.17 pg/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Epigenetics and Nuclear Signaling
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of mouse PADI4 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	91
	Range %	85-99
1:2	Average %	95
	Range %	89-101
1:4	Average %	96
	Range %	92-110
1:8	Average %	93
	Range %	86-102

回收率：

The recovery of mouse PADI4 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	97	88-105
EDTA plasma (n=4)	96	89-104

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
300	2.228	2.230	2.229	2.051
150	1.457	1.378	1.418	1.240
75	0.854	0.822	0.838	0.660
37.5	0.483	0.513	0.498	0.320
18.75	0.355	0.363	0.359	0.181
9.4	0.274	0.259	0.267	0.089
4.7	0.233	0.226	0.230	0.052
0	0.175	0.181	0.178

數據處理：

ELISA操作視頻及標曲繪制軟件下載
貨期：

3-5 working days

Ferroptosis Induces gut microbiota and metabolic dysbiosis in Collagen-Induced arthritis mice via PAD4 enzyme X Zhu, H Lu, W Li, S Niu, J Xue, H Sun, J Zhang,Gene,2024

功能：

Catalyzes the citrullination/deimination of arginine residues of proteins such as histones, thereby playing a key role in histone code and regulation of stem cell maintenance. Citrullinates histone H1 at 'Arg-54' (to form H1R54ci), histone H3 at 'Arg-2', 'Arg-8', 'Arg-17' and/or 'Arg-26' (to form H3R2ci, H3R8ci, H3R17ci, H3R26ci, respectively) and histone H4 at 'Arg-3' (to form H4R3ci). Acts as a key regulator of stem cell maintenance by mediating citrullination of histone H1: citrullination of 'Arg-54' of histone H1 (H1R54ci) results in H1 displacement from chromatin and global chromatin decondensation, thereby promoting pluripotency and stem cell maintenance. Promotes profound chromatin decondensation during the innate immune response to infection in neutrophils by mediating formation of H1R54ci. Required for the formation of neutrophil extracellular traps (NETs); NETs are mainly composed of DNA fibers and are released by neutrophils to bind pathogens during inflammation. Citrullination of histone H3 prevents their methylation by CARM1 and HRMT1L2/PRMT1 and represses transcription. Citrullinates EP300/P300 at 'Arg-2142', which favors its interaction with NCOA2/GRIP1.
基因功能參考文獻：
1. PAD4 in neutrophils is essential for kidney NET formation and ischemic kidney injury. PMID: 29061334
2. PADi4 is critical for neutrophil extracellular traps formation, but is dispensable for generalized citrullination PMID: 28188029
3. sFlt-1 overexpression in Padi4(-/-) mice resulted in dramatically lower inflammatory and thrombotic response, which was accompanied by significant reduction in pregnancy losses. Inhibition of NETosis may serve as a novel target in disorders of impaired placentation. PMID: 28007693
4. a role for NETs in cardiac fibrosis and conclude that PAD4 regulates age-related organ fibrosis and dysfunction. PMID: 28031479
5. PADI4 exacerbates arthritis with diverse immunological modifications. PMID: 26293116
6. Selective inhibition of peptidylarginine deiminase IV with Cl-amidine blocked NET formation, reduced atherosclerotic lesion area, and delayed time to carotid artery thrombosis in a photochemical injury model. PMID: 24425713
7. Padi4 is part of the pluripotency transcriptional network, binding to regulatory elements of key stem-cell genes and activating their expression PMID: 24463520
8. PAD4-mediated chromatin decondensation in the neutrophil is crucial for pathological venous thrombosis and present neutrophil activation and PAD4 as potential drug targets for deep vein thrombosis. PMID: 23650392
9. PAD4 is dispensable in this effector phase model of arthritis PMID: 22551352
10. analysis of regulation of histone modification and chromatin structure by the p53-PADI4 pathway PMID: 22334079
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亞細胞定位：

Cytoplasm. Nucleus. Cytoplasmic granule.
蛋白家族：

Protein arginine deiminase family
組織特異性：

Expressed in pluripotent embryonic stem and induced pluripotent stem cells but not multipotent neural stem cells.
數據庫鏈接：

KEGG: mmu:18602

STRING: 10090.ENSMUSP00000026381

UniGene: Mm.250358

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.