Mouse Phospholipid hydroperoxide glutathione peroxidase, mitochondrial(GPX4) ELISA kit

產品詳情

文獻引用 (2) 說明書下載

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產品描述：

小鼠谷胱甘肽過氧化物酶，線粒體(GPX4)酶聯免疫試劑盒（CSB-EL009869MO）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿、細胞裂解物樣本中的GPX4含量。GPX4是鐵死亡過程中的關鍵調節(jié)因子，通過將GSH轉化為GSSH來限制脂質過氧化，保護細胞免受鐵死亡。其表達受NRF2調控，在多種腫瘤治療中具有潛在應用價值。試劑盒檢測范圍為31.25 pg/mL-2000 pg/mL，適用于科研領域中對GPX4蛋白表達的動態(tài)監(jiān)測，如鐵死亡機制研究、抗氧化藥物篩選、疾病模型構建及相關信號通路探索。為細胞生物學、基礎醫(yī)學研究提供靈敏穩(wěn)定的分析工具。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
縮寫：

GPX4
Uniprot No.：

Q91XR9
種屬：

Mus musculus (Mouse)
樣本類型：

serum, plasma, tissue homogenates, cell lysates
檢測范圍：

31.25 pg/mL-2000 pg/mL
靈敏度：

7.81 pg/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Cancer
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of mouse GPX4 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	90
	Range %	86-95
1:2	Average %	100
	Range %	97-104
1:4	Average %	94
	Range %	85-97
1:8	Average %	98
	Range %	91-103

回收率：

The recovery of mouse GPX4 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	89-98
EDTA plasma (n=4)	92	87-98

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.