Mouse Neuron-specific enolase,NSE ELISA Kit

Mouse Neuron-specific enolase,NSE ELISA Kit

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文獻引用 (2) 說明書下載

中文名稱：

小鼠神經(jīng)特異性烯醇化酶(NSE)酶聯(lián)免疫試劑盒
貨號：

CSB-E07962m
規(guī)格：

96T/48T
價格：

￥3800/￥2500
其他：

CUSABIO Wins 2024 Bioz Rapid Star? Award in the ELISA category

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產(chǎn)品描述：

小鼠神經(jīng)特異性烯醇化酶(NSE)酶聯(lián)免疫試劑盒（CSB-E07962m）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織培養(yǎng)上清液樣本中的ENO2含量。ENO2作為靶點，它是烯醇化酶的一種。ENO2在神經(jīng)組織及神經(jīng)內(nèi)分泌來源的細胞中特異性表達，在腫瘤發(fā)生發(fā)展等過程可能發(fā)揮重要作用。其研究機制主要圍繞探究其在細胞代謝、增殖、凋亡等方面的分子調(diào)控作用，以尋找潛在治療靶點和干預(yù)手段。試劑盒檢測范圍為1.56 ng/mL-100 ng/mL，適用于神經(jīng)損傷模型評估、神經(jīng)細胞分化研究、神經(jīng)相關(guān)疾病機制探索以及藥物干預(yù)效果監(jiān)測等科研領(lǐng)域，為神經(jīng)科學(xué)基礎(chǔ)研究提供穩(wěn)定可靠的工具支持。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
別名：

Eno2 ELISA Kit; Eno-2Gamma-enolase ELISA Kit; EC 4.2.1.11 ELISA Kit; 2-phospho-D-glycerate hydro-lyase ELISA Kit; Enolase 2 ELISA Kit; Neural enolase ELISA Kit; Neuron-specific enolase ELISA Kit; NSE ELISA Kit
縮寫：

ENO2
Uniprot No.：

P17183
種屬：

Mus musculus (Mouse)
樣本類型：

serum, plasma, cell culture supernates
檢測范圍：

1.56 ng/mL-100 ng/mL
靈敏度：

0.39 ng/mL
反應(yīng)時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領(lǐng)域：

Metabolism
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of mouse NSE in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	98
	Range %	89-102
1:2	Average %	91
	Range %	87-95
1:4	Average %	94
	Range %	84-97
1:8	Average %	91
	Range %	85-94

回收率：

The recovery of mouse NSE spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	105	92-108
EDTA plasma (n=4)	91	84-98

標(biāo)準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
100	2.622	2.542	2.582	2.475
50	2.209	2.167	2.188	2.081
25	1.463	1.487	1.475	1.368
12.5	0.762	0.734	0.748	0.641
6.25	0.358	0.333	0.346	0.239
3.12	0.252	0.245	0.249	0.142
1.56	0.180	0.168	0.174	0.067
0	0.106	0.108	0.107

數(shù)據(jù)處理：

ELISA操作視頻及標(biāo)曲繪制軟件下載
貨期：

3-5 working days

Combined Effects of Particulate Matter Exposure and Exercise Training on Neuroplasticity-Related Growth Factors and Blood–Brain Barrier Integrity SY Cho, HT Roh,Atmosphere,2025
Dephosphorylation by calcineurin regulates translocation of dynamin-related protein 1 to mitochondria in hepatic ischemia reperfusion induced hippocampus injury in young mice Yu X, et al,Brain Research,2019

功能：

Has neurotrophic and neuroprotective properties on a broad spectrum of central nervous system (CNS) neurons. Binds, in a calcium-dependent manner, to cultured neocortical neurons and promotes cell survival.
基因功能參考文獻：
1. Study analyzed the regions that regulate the promoter strength and cellular specificity of the 1.8 kb rNSE promoter in the cerebellum in vivo. The rNSE promoter was divided into three regions (A, B, and C). Our results showed that region C was indispensable for the promoter activity in mouse cerebellum and served as an interneuron-predominant basal promoter. PMID: 28508170
2. Plaque-associated microglial cells express gamma-enolase and cathepsin X. PMID: 23621429
3. Treadmill exercise training decreased the expression of NSN in the striatum of chronic Parkinsonian mice, which can partially explain the beneficial neuroprotective role of exercise in patients with parkinson disease. PMID: 21725169
4. we induced differentiation of mouse stromal cells to neuron-like cells and observed the activation of tissue-specific promoter of neuron-specific enolase in transgenic mice with the human bcl-2 gene expressed only in neurons under the NSE promoter PMID: 14593212
亞細胞定位：

Cytoplasm. Cell membrane.
蛋白家族：

Enolase family
組織特異性：

Skeletal muscle (at protein level). The alpha/alpha homodimer is expressed in embryo and in most adult tissues. The alpha/beta heterodimer and the beta/beta homodimer are found in striated muscle, and the alpha/gamma heterodimer and the gamma/gamma homodi
數(shù)據(jù)庫鏈接：

KEGG: mmu:13807

STRING: 10090.ENSMUSP00000004378

UniGene: Mm.3913

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.