Mouse N-acetyl-β-D-glucosaminidase,NAG ELISA Kit

Mouse N-acetyl-β-D-glucosaminidase,NAG ELISA Kit

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中文名稱：

小鼠N-乙酰-β-D-氨基葡萄糖苷酶(NAG)酶聯免疫試劑盒
貨號：

CSB-E07444m
規格：

96T/48T
價格：

￥3600/￥2500
其他：

CUSABIO Wins 2024 Bioz Rapid Star? Award in the ELISA category

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產品描述：

小鼠N-乙酰-β-D-氨基葡萄糖苷酶(NAG)酶聯免疫試劑盒（CSB-E07444m）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的NAG含量。試劑盒檢測范圍為15.6 μIU/mL-1000 μIU/mL，適用于基礎醫學研究中NAG相關代謝通路探索、藥物腎毒性評估、腎臟疾病動物模型構建等科研領域，特別適合用于分析腎小管損傷模型或肝臟代謝研究中NAG的動態變化。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
別名：

Hexb ELISA Kit; Beta-hexosaminidase subunit beta ELISA Kit; EC 3.2.1.52 ELISA Kit; Beta-N-acetylhexosaminidase subunit beta ELISA Kit; Hexosaminidase subunit B ELISA Kit; N-acetyl-beta-glucosaminidase subunit beta ELISA Kit
縮寫：

NAG
Uniprot No.：

P20060
種屬：

Mus musculus (Mouse)
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

15.6 μIU/mL-1000 μIU/mL
靈敏度：

3.9 μIU/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Others
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of mouse NAG in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:10	Average %	93
	Range %	85-99
1:20	Average %	95
	Range %	92-98
1:40	Average %	90
	Range %	87-100
1:80	Average %	88
	Range %	86-98

回收率：

The recovery of mouse NAG spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	97	86-101
EDTA plasma (n=4)	95	90-98

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

μIU/ml	OD1	OD2	Average	Corrected
1000	2.555	2.529	2.542	2.398
500	2.084	2.105	2.095	1.951
250	1.442	1.467	1.455	1.311
125	0.946	0.955	0.951	0.807
62.5	0.413	0.408	0.411	0.267
31.2	0.321	0.326	0.324	0.180
15.6	0.244	0.239	0.242	0.098
0	0.142	0.145	0.144

數據處理：

ELISA操作視頻及標曲繪制軟件下載
貨期：

3-5 working days

Ursodeoxycholic acid protects against sepsis-induced acute kidney injury by activating Nrf2/HO-1 and inhibiting NF-κB pathway Y Lou, H Shi, N Sha, F Li, X Gu, H Lin,BMC nephrology,2025
Wnt9a Promotes Renal Fibrosis by Accelerating Cellular Senescence in Tubular Epithelial Cells Luo C,Journal of the American Society of Nephrology,2023
Sirtuin-1 attenuates cadmium-induced renal cell senescence through p53 deacetylation X Chou,Ecotoxicology and Environmental Safety,2022
Sirtuin-1 attenuates cadmium-induced renal cell senescence through p53 deacetylation X Chou,Ecotoxicology and environmental safety,2022
Ambient fine particulate matter induced the elevation of blood pressure through ACE2/Ang(1–7) pathway: The evidence from urine metabolites X Du,Ecotoxicology and Environmental Safety,2020

功能：

Hydrolyzes the non-reducing end N-acetyl-D-hexosamine and/or sulfated N-acetyl-D-hexosamine of glycoconjugates, such as the oligosaccharide moieties from proteins and neutral glycolipids, or from certain mucopolysaccharides. The isozyme B does not hydrolyze each of these substrates, however hydrolyzes efficiently neutral oligosaccharide. Only the isozyme A is responsible for the degradation of GM2 gangliosides in the presence of GM2A. During fertilization is responsible, at least in part, for the zona block to polyspermy. Present in the cortical granules of non-activated oocytes, is exocytosed during the cortical reaction in response to oocyte activation and inactivates the sperm galactosyltransferase-binding site, accounting for the block in sperm binding to the zona pellucida.
基因功能參考文獻：
1. mast cell granule beta-hexosaminidase is crucial for defense against bacterial invasion, but is not involved in the allergic response. the bactericidal mechanism of beta-hexosaminidase involves degradation of bacterial cell wall peptidoglycan. PMID: 25015817
2. transgenic inducible strains of Sandhoff disease mice provide a dynamic platform with which to explore the pathophysiological sequelae immediately after loss of neuronal lysosomal beta-hexosaminidase activity. PMID: 23028353
3. Thymic involution and corticosterone level in Sandhoff disease model mice. PMID: 21598013
4. These data suggest that the restricted ketogenic diet and N-butyldeoxynojirimycin may be a potential combinatorial therapy for Sandhoff disease. PMID: 20374428
5. Data suggest that pathogenesis of Sandhoff disease (heritable beta-hexosaminidase deficiency) involves an increase in macrophage-inflammatory protein 1alpha that induces monocytes to infiltrate the CNS and trigger neuronal apoptosis. PMID: 15155903
6. The bicistronic beta-hexosaminidase vector can reverse the biochemical defects and down-stream consequences in Sandhoff neurons, reinforcing its potential for Sandhoff disease in vivo gene therapy. PMID: 16441513
7. Beta-hexosaminidase is a peptidoglycan hydrolase that surprisingly exerts its mycobactericidal effect at the macrophage plasma membrane during mycobacteria-induced secretion of lysosomes PMID: 18180457
8. There was no change in the level of GM2 storage and pro-apoptotic activity or astrocyte activation in HexB-/- knockout mice PMID: 18657867
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亞細胞定位：

Lysosome. Cytoplasmic vesicle, secretory vesicle, Cortical granule.
蛋白家族：

Glycosyl hydrolase 20 family
數據庫鏈接：

KEGG: mmu:15212

STRING: 10090.ENSMUSP00000022169

UniGene: Mm.27816

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.