Mouse Monocyte Chemotactic Protein 5 (MCP-5) ELISA Kit

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中文名稱：

小鼠單核細胞趨化蛋白5(MCP-5)酶聯(lián)免疫試劑盒
貨號：

CSB-E15766m
規(guī)格：

96T/48T
價格：

￥3600/￥2500
其他：

CUSABIO Wins 2024 Bioz Rapid Star? Award in the ELISA category

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產(chǎn)品描述：

小鼠單核細胞趨化蛋白5(MCP-5)酶聯(lián)免疫試劑盒（CSB-E15766m）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的MCP-5含量。MCP - 5是一種關鍵靶點。它在免疫調(diào)節(jié)等生理過程中發(fā)揮重要作用，可能參與炎癥反應等病理進程。目前研究聚焦于其在疾病發(fā)生發(fā)展中的機制，了解它與免疫細胞的相互作用、信號傳導通路等，為開發(fā)針對性治療方法提供依據(jù)。試劑盒檢測范圍為15.6 pg/mL-1000 pg/mL，靈敏度為16.82 pg/mL。適用于科研場景下對小鼠炎癥相關模型、免疫調(diào)節(jié)機制或藥物干預效果的研究，例如通過檢測血清中MCP - 5水平評估炎癥程度，或分析組織勻漿中趨化因子表達變化以探究疾病發(fā)生機制，為免疫學、藥理學及病理學研究提供可靠工具。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
別名：

C-C motif chemokine 12 ELISA Kit; Ccl12 ELISA Kit; CCL12_MOUSE ELISA Kit; Macrophage/monocyte Chemotactic Protein ELISA Kit; MCP-1-related chemokine ELISA Kit; MCP-5 ELISA Kit; Monocyte chemoattractant protein 5 ELISA Kit; Monocyte chemotactic protein 5 ELISA Kit; OTTMUSP00000000792 ELISA Kit; Scya12 ELISA Kit; Small inducible cytokine A12 ELISA Kit; Small-inducible cytokine A12 ELISA Kit
縮寫：

MCP-5
Uniprot No.：

Q62401
種屬：

Mus musculus (Mouse)
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

15.6 pg/mL-1000 pg/mL
靈敏度：

16.82 pg/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Immunology
測定原理：

quantitative
測定方法：

Sandwich

精密度：

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of mouse MCP-5 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	87
	Range %	82-91
1:2	Average %	94
	Range %	89-98
1:4	Average %	107
	Range %	103-110
1:8	Average %	110
	Range %	104-114

回收率：

The recovery of mouse MCP-5 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	91-99
EDTA plasma (n=4)	103	98-108

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
1000	2.615	2.634	2.625	2.499
500	2.177	2.012	2.095	1.969
250	1.432	1.502	1.467	1.341
125	0.842	0.839	0.841	0.715
62.5	0.531	0.529	0.530	0.404
31.2	0.334	0.326	0.330	0.204
15.6	0.246	0.251	0.249	0.123
0	0.125	0.127	0.126

數(shù)據(jù)處理：

ELISA操作視頻及標曲繪制軟件下載
貨期：

3-5 working days

引用文獻

The Effects of Interleukin-17 (IL-17)-Related Inflammatory Cytokines and A20 Regulatory Proteins on Astrocytes in Spinal Cord Cultured In Vitro Zong S.et al,Cell Physiol Biochem.,2016

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最新研究進展：

MCP-5是一種趨化因子，全名為單核細胞趨化蛋白5。它主要參與免疫細胞的趨化和活化過程，在炎癥、感染、腫瘤等疾病的發(fā)生和發(fā)展中發(fā)揮重要作用。最新研究發(fā)現(xiàn)，MCP-5參與了多種腫瘤的發(fā)生和進展，同時也是一種重要的治療靶點。
功能：

Chemotactic factor that attracts eosinophils, monocytes, and lymphocytes but not neutrophils. Potent monocyte active chemokine that signals through CCR2. Involved in allergic inflammation and the host response to pathogens and may play a pivotal role during early stages of allergic lung inflammation.
基因功能參考文獻：
1. After 100% O2 treatment, genes involved in inflammation (Ccl12), angiogenesis (Igfr1, Stat3), and metabolism (Hk2) were upregulated. PMID: 24458145
2. Data indicate mRNA upregulation of CCR2 and CCR2 ligands CCL2, CCL7, CCL8, and CCL12 in aortas from DOCA/salt-treated hypertension. PMID: 23033370
3. The transforming growth factor (TGF)beta type II receptor/MCP-5 axis is an essential crossroad for joint development and endochondral growth. PMID: 22814601
4. CCL12 proteins were present in the vast majority of tissues investigated. mRNA for these proteins was also expressed in most of these tissues suggesting local production and the ability to respond in situ to inflammatory stimuli. PMID: 20931267
亞細胞定位：

Secreted.
蛋白家族：

Intercrine beta (chemokine CC) family
組織特異性：

Predominantly expressed in the lymph nodes and thymus. Also found in the salivary glands containing lymph nodes, breast, heart, lung, brain, small intestine, kidney and colon.
數(shù)據(jù)庫鏈接：

KEGG: mmu:20293

STRING: 10090.ENSMUSP00000000194

UniGene: Mm.867

Mouse Monocyte Chemotactic Protein 5 (MCP-5) ELISA Kit

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