Mouse Metallothionein-2(MT-2) ELISA Kit

Mouse Metallothionein-2(MT-2) ELISA Kit

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中文名稱：

小鼠金屬硫蛋白2(MT-2)酶聯(lián)免疫試劑盒
貨號：

CSB-E13693m
規(guī)格：

96T/48T
價格：

￥3600/￥2500
其他：

CUSABIO Wins 2024 Bioz Rapid Star? Award in the ELISA category

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小鼠金屬硫蛋白2(MT-2)酶聯(lián)免疫試劑盒（CSB-E13693m）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的MT-2含量。試劑盒檢測范圍為15.6 pg/mL-1000 pg/mL，適用于小鼠模型中的金屬代謝異常、氧化應激相關疾病機制研究，以及重金屬暴露實驗、肝臟損傷模型或環(huán)境毒理學研究中的生物標志物分析本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
別名：

Mt2 ELISA Kit; Metallothionein-2 ELISA Kit; MT-2 ELISA Kit; Metallothionein-II ELISA Kit; MT-II ELISA Kit
縮寫：

MT-2
Uniprot No.：

P02798
種屬：

Mus musculus (Mouse)
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

15.6 pg/mL-1000 pg/mL
靈敏度：

3.9 pg/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Epigenetics and Nuclear Signaling
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of mouse MT-2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	96
	Range %	91-102
1:2	Average %	102
	Range %	99-105
1:4	Average %	89
	Range %	86-93
1:8	Average %	94
	Range %	92-97

回收率：

The recovery of mouse MT-2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	103	101-106
EDTA plasma (n=4)	95	92-109

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
1000	1.975	2.022	1.999	1.842
500	1.362	1.377	1.370	1.213
250	0.811	0.862	0.837	0.680
125	0.433	0.439	0.436	0.279
62.5	0.304	0.330	0.317	0.160
31.2	0.262	0.274	0.268	0.111
15.6	0.214	0.220	0.217	0.060
0	0.163	0.150	0.157

數(shù)據(jù)處理：

ELISA操作視頻及標曲繪制軟件下載
貨期：

3-5 working days

功能：

Metallothioneins have a high content of cysteine residues that bind various heavy metals; these proteins are transcriptionally regulated by both heavy metals and glucocorticoids.
基因功能參考文獻：
1. these results demonstrate that MT2 plays a role in CD4+ T cell activation by transducing reactive oxygen species signals into an increased [Zn2+]i that subsequently affects downstream effector function PMID: 27251638
2. Metallothioneins may have a protective role against post-burn inflammation and inflammatory organ damage, at least partly through inhibiting the p38 MAPK signaling. PMID: 26833479
3. The MT2 receptor activates Akt/GSK-3beta/CRMP-2 signaling and is necessary and sufficient to mediate functional axonogenesis and synaptic formation in central neurons. PMID: 25501601
4. Zn deficiency in utero induces fetal epigenetic alterations of Mt2 gene and these changes are being stored as an epigenetic memory until adulthood. PMID: 22917840
5. Knockdown of metallothionein 2 does not affect atrophy or oxidant activity in skeletal myotubes subject to serum reduction. PMID: 20798270
6. MT-null phenotype precludes lead-induced inclusion body formation and increases lead toxicity at the organ and cellular level despite reducing lead accumulation PMID: 11891201
7. MT-2 deficiency increases brain pathology in transgenic mice with astrocyte-targeted expression of interleukin 6. PMID: 11950277
8. In mouse spinal cord injury lead the up-regulation MT-2 expression at 1 day and MT-2 expression was observed in activated macrophages/microglia at 1 day. PMID: 15592854
9. MT2a has a role in oxidative stress, and the formation of MT disulfide bonds is involved in the regulation of zinc homeostasis PMID: 16243847
10. Mt-2 synthesis was induced by cilostazol. PMID: 17409522
11. Metallothionein II deficient mice exhibit impaired rate of maze learning but retained the information as well as the wild-type mice. The absence of functional MT-II does not result in significantly worse injury following 45 min of hypoxia-ischemia. PMID: 18083145
12. During the early phase of learning metallothionein-2 null mice were more susceptible to mercury-induced impairment compared to wildtype mice PMID: 18226494
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蛋白家族：

Metallothionein superfamily, Type 1 family
數(shù)據(jù)庫鏈接：

KEGG: mmu:17750

STRING: 10090.ENSMUSP00000034214

UniGene: Mm.147226

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.