Mouse Membrane primary amine oxidase(AOC3) ELISA kit

Mouse Membrane primary amine oxidase(AOC3) ELISA kit

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中文名稱：

小鼠伯胺氧化酶膜(AOC3)酶聯免疫試劑盒
貨號：

CSB-EL001855MO
規格：

96T/48T
價格：

￥3600/￥2500
其他：

CUSABIO Wins 2024 Bioz Rapid Star? Award in the ELISA category

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產品描述：

小鼠伯胺氧化酶膜(AOC3)酶聯免疫試劑盒（CSB-EL001855MO）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的AOC3含量。AOC3作為靶點，是含氨氧化酶家族成員，主要分布在白細胞等細胞表面。其作用機制與細胞黏附、遷移等過程相關。目前研究聚焦于其在免疫調節、炎癥反應等病理生理過程中的角色，有望為相關疾病的治療提供新方向。試劑盒檢測范圍為1.25 ng/mL-80 ng/mL，本試劑盒適用于科研場景下的小鼠模型研究，如通過檢測血清或血漿樣本評估循環系統中AOC3的動態變化，或通過組織勻漿分析特定器官（如肝臟、脂肪組織）中該蛋白的表達特征，為炎癥機制、藥物干預效果或免疫調節研究提供定量分析工具。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
別名：

Aoc3; Vap1; Membrane primary amine oxidase; Copper amine oxidase; Semicarbazide-sensitive amine oxidase; SSAO; Vascular adhesion protein 1; VAP-1
縮寫：

AOC3
Uniprot No.：

O70423
種屬：

Mus musculus (Mouse)
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

1.25 ng/mL-80 ng/mL
靈敏度：

0.31 ng/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Others
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of mouse AOC3 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	83
	Range %	80-86
1:2	Average %	90
	Range %	85-96
1:4	Average %	96
	Range %	93-99
1:8	Average %	104
	Range %	100-109

回收率：

The recovery of mouse AOC3 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	90-100
EDTA plasma (n=4)	97	94-100

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
80	2.507	2.569	2.538	2.351
40	1.662	1.600	1.631	1.444
20	0.999	0.968	0.984	0.797
10	0.587	0.572	0.580	0.393
5	0.444	0.458	0.451	0.264
2.5	0.354	0.362	0.358	0.171
1.25	0.279	0.298	0.289	0.102
0	0.188	0.186	0.187

數據處理：

ELISA操作視頻及標曲繪制軟件下載
貨期：

3-5 working days

功能：

Cell adhesion protein that participates in lymphocyte recirculation by mediating the binding of lymphocytes to peripheral lymph node vascular endothelial cells in an L-selectin-independent fashion. Has a monoamine oxidase activity. May play a role in adipogenesis.
基因功能參考文獻：
1. Data indicate strong-to-moderate expression of vascular adhesion protein-1 (VAP-1) in the synovium of Borrelia burgdorferi-infected mice, while only weak expression of VAP-1 was detected in uninfected mice. PMID: 29166944
2. VAP-1 was expressed on endothelial cells lining inflamed atherosclerotic lesions; normal vessel walls in aortas of C57BL/6N control mice were VAP-1-negative. PMID: 27731409
3. VAP-1 plays a critical role in the pathophysiology of renal ischemia/reperfusion injury by enhancement of neutrophil infiltration generating a local hydrogen peroxide gradient. PMID: 28318627
4. This study showed that the oxidase activity of AOC3 contributes to the development of lung fibrosis mainly by regulating the accumulation of pathogenic leukocyte subtypes, which drive the fibrotic response. PMID: 28251930
5. data indicate that SSAO inactivation in vivo stabilizes the established plaques mainly via inducing the switch of SMCs from a contractile to a synthetic phenotype PMID: 27043821
6. Suggest inhibiting VAP-1/SSAO enzymatic function, by PXS-4728A, as a novel therapeutic approach in lung diseases that are characterized by neutrophilic pattern of inflammation. PMID: 25889951
7. these results link the amine oxidase activity of VAP-1 with hepatic inflammation and fibrosis and suggest that targeting VAP-1 has therapeutic potential for NAFLD and other chronic fibrotic liver diseases. PMID: 25562318
8. these results suggest a largely redundant function for AOC3/VAP-1 in allergic inflammatory responses of the airways. PMID: 25116373
9. The results of the study establish VCAM-1 and VAP-1 as mediators of myeloid cell recruitment in metastasis and identify VAP-1 as a potential target for therapeutic intervention to combat early metastasis. PMID: 23407548
10. The antihyperglycemic effect of benzylamine is abolished by AOC3 gene knockout in mice. PMID: 22547093
11. Long-term activation of semicarbazide-sensitive amine oxidase by benzylamine lowers circulating levels of uric acid in diabetic conditions. PMID: 22480418
12. AOC3 expression is increased in perigonadal and subcutaneous adipose tissue of diabetic db-/- obese mice. PMID: 21298297
13. This review examines the biological functions of VAP-1, especially the role that this molecule might play in the establishment and progression of chronic liver disease. PMID: 21512782
14. The demonstrated AOC3 turnover of primary amines that are non-native to human tissue suggests possible roles for the adipocyte enzyme in subcutaneous bacterial infiltration and obesity. PMID: 22238597
15. VAP-1-mediated M2 macrophage infiltration underlies IL-1beta- but not VEGF-A-induced lymph- and angiogenesis PMID: 21435467
16. VAP-1 inhibition provided antiinflammation effect by reducing adhesion molecule expression and immune cell infiltration after intracerebral hemorrhage. PMID: 20877383
17. histamine moderately activated lipolysis in adipocytes in AOC3 knockout mice PMID: 20012150
18. Enzyme is in involved in deamination in atherogenesis in KKAy diabetic mice fed with high cholesterol diet. PMID: 12242458
19. the increased enzyme activity observed in diabetes is not a result of increased SSAO gene transcription. It is controlled by posttranslational modifications of the protein, and the catalytic activity controls the gene expression PMID: 12606817
20. vascular adhesion protein-1 expression may contribute to the functional heterogeneity of endothelial cells within the lung to create distinct sites for the recruitment of inflammatory cells PMID: 12700900
21. Release of this enzyme is regulated by TNF-alpha and insulin and, in adipose cells could contribute to the atherogenesis and vascular dysfunction associated with diabetes and obesity. PMID: 14968297
22. The vascular endothelium is the major source of circulating VAP-1 protein and semicarbazide-sensitive amine oxidase activity. PMID: 15178639
23. VAP-1 mediates leukocyte trafficking to sites of inflammation and thus is a potential target for anti-inflammatory therapies PMID: 15743791
24. T helper type 2 cells use Vap-1 to roll and adhere in the inflamed liver microcirculation PMID: 16111634
25. These data demonstrate the potential clinical benefit of small molecule anti-SSAO therapy in this mouse multiple sclerosis model. PMID: 17393060
26. The effects of exogenous or biogenic amines on glucose transport are not receptor-mediated but are oxidation-dependent. The major SSAO form expressed in mouse adipocytes is encoded by the AOC3 gene. PMID: 17406965
27. VAP-1-deficient mice have mild deviations in the mucosal immune system PMID: 17947691
28. The atheroprotective effect of LXR agonist T0901317 is related to the inhibition of SSAO gene expression and its activity. PMID: 18330481
29. LOX binds to mature TGF-beta1 and enzymatically regulates its signaling in bone via amine oxidase activity, and thus may play an important role in bone maintenance and remodeling PMID: 18835815
30. VAP-1 regulates the inflammatory response in ischemia-reperfusion injury and suggest that blockade of VAP-1 may have therapeutic value. PMID: 18991279
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亞細胞定位：

Membrane; Single-pass type II membrane protein.
蛋白家族：

Copper/topaquinone oxidase family
數據庫鏈接：

KEGG: mmu:11754

STRING: 10090.ENSMUSP00000099394

UniGene: Mm.67281

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.