Mouse Melanoma-derived growth regulatory protein(MIA) ELISA kit

Mouse Melanoma-derived growth regulatory protein(MIA) ELISA kit

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中文名稱：

小鼠黑色素瘤源性生長調控蛋白(MIA)酶聯免疫試劑盒
貨號：

CSB-EL013798MO
規格：

96T/48T
價格：

￥3600/￥2500
其他：

CUSABIO Wins 2024 Bioz Rapid Star? Award in the ELISA category

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產品描述：

小鼠黑色素瘤源性生長調控蛋白(MIA)酶聯免疫試劑盒（CSB-EL013798MO）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的MIA含量。試劑盒檢測范圍為4.7 pg/mL-300 pg/mL，適用于科研領域中對黑色素瘤動物模型的研究，如腫瘤微環境分析、轉移機制探索、藥物干預效果評估等場景，為揭示MIA在腫瘤生物學中的作用及開發相關治療策略提供可靠工具。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
別名：

Mia ELISA Kit; Cdrap ELISA Kit; Mia1 ELISA Kit; Melanoma-derived growth regulatory protein ELISA Kit; Cartilage-derived retinoic acid-sensitive protein ELISA Kit; CD-RAP ELISA Kit; Melanoma inhibitory activity protein ELISA Kit
縮寫：

MIA
Uniprot No.：

Q61865
種屬：

Mus musculus (Mouse)
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

4.7 pg/mL-300 pg/mL
靈敏度：

1.17 pg/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Cancer
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of mouse MIA in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:100	Average %	96
	Range %	82-99
1:200	Average %	101
	Range %	97-105
1:400	Average %	92
	Range %	88-96
1:800	Average %	87
	Range %	85-89

回收率：

The recovery of mouse MIA spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	104	101-107
EDTA plasma (n=4)	96	92-99

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
300	2.502	2.563	2.533	2.426
150	2.256	2.212	2.234	2.127
75	1.698	1.680	1.689	1.582
37.5	0.859	0.899	0.879	0.772
18.75	0.514	0.536	0.525	0.418
9.4	0.301	0.326	0.314	0.207
4.7	0.192	0.172	0.182	0.075
0	0.107	0.106	0.107

數據處理：

ELISA操作視頻及標曲繪制軟件下載
貨期：

3-5 working days

功能：

Elicits growth inhibition on melanoma cells in vitro as well as some other neuroectodermal tumors, including gliomas.
基因功能參考文獻：
1. The effects of MIA/CD-RAP on transcriptional regulation in chondrocytes, through the regulation of p54(nrb) via YBX1 contributes to the understanding of chondrogenesis. PMID: 24349210
2. Attenuated LM expressing MIA. PMID: 22886976
3. as observed in other knockout models of molecules important for cartilage development and differentiation, viability and functional integrity is reached by remarkable molecular redundancy in MIA/CD-RAP knockout mice. PMID: 24269712
4. The cartilage protein melanoma inhibitory activity contributes to inflammatory arthritis. PMID: 24287514
5. Expression analysis of cartilage tissue derived from MIA-/- mice revealed strong downregulation of nuclear RNA-binding protein 54-kDa. PMID: 21368873
6. MIA/CD-RAP stabilizes cartilage differentiation and inhibits differentiation into bone potentially by regulating signaling processes during differentiation. PMID: 20164682
7. transcriptional mechanism by which CD-RAP expression is suppressed by IL-1 beta PMID: 12072435
8. Cdrap/Mia is located between two housekeeping genes PMID: 15028289
9. DNA promoter segment from -2,251 bp to -2,068 bp of the CD-RAP gene contains elements critical for gene expression. It demonstrates activation or repression of gene expression in vitro and in vivo based on cell type and content of transcription factors. PMID: 16250001
10. A potential tumor suppressor role of Mia/Cd-rap in pituitary cells. PMID: 17914116
11. Microarray analysis showed significant reductions in the expression of several proteins in lung misexpressing MIA. PMID: 18342301
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亞細胞定位：

Secreted.
蛋白家族：

MIA/OTOR family
組織特異性：

All malignant melanoma cell lines tested and infrequently in glioma cell lines.
數據庫鏈接：

KEGG: mmu:12587

UniGene: Mm.262447

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.