Mouse Mast Cell Tryptase(MCT) ELISA Kit

產品詳情

文獻引用 (6) 說明書下載

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產品描述：

小鼠肥大細胞類胰蛋白酶(MCT)酶聯免疫試劑盒（CSB-E14326m）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織培養上清液、組織勻漿樣本中的MCT含量。MCT（單羧酸轉運體）是重要的細胞膜轉運蛋白。背景上，它在多種細胞代謝過程中起關鍵作用。研究機制方面，主要負責轉運單羧酸類物質如乳酸等，在能量代謝、細胞內pH調節等過程有重要意義，其功能異常與腫瘤等疾病發展相關。試劑盒檢測范圍為31.25 pg/mL-2000 pg/mL，廣泛應用于科研領域，如肥大細胞活化機制研究、過敏性動物模型評估、炎癥相關藥物篩選等場景，為探索MCT在免疫調控中的作用提供可靠工具。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
別名：

Tpsab1 ELISA Kit; Mcpt7 ELISA Kit; Tryptase ELISA Kit; EC 3.4.21.59 ELISA Kit; Mast cell protease 7 ELISA Kit; mMCP-7 ELISA Kit; Tryptase alpha/beta-1 ELISA Kit
縮寫：

MCT
Uniprot No.：

Q02844
種屬：

Mus musculus (Mouse)
樣本類型：

serum, plasma, cell culture supernates, tissue homogenates
檢測范圍：

31.25 pg/mL-2000 pg/mL
靈敏度：

7.8 pg/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Signal Transduction
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of mouse MCT in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	92
	Range %	89-96
1:2	Average %	96
	Range %	91-102
1:4	Average %	97
	Range %	93-100
1:8	Average %	93
	Range %	88-99

回收率：

The recovery of mouse MCT spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	96	93-101
EDTA plasma (n=4)	91	84-97

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

功能：

Tryptase is the major neutral protease present in mast cells and is secreted upon the coupled activation-degranulation response of this cell type. May play a role in innate immunity.
基因功能參考文獻：
1. transcriptional activation of mouse mast cell Protease-7 by activin and transforming growth factor-beta is inhibited by microphthalmia-associated transcription factor PMID: 14527958
2. Because mMCP-6 and mMCP-7 can compensate for each other in this mouse disease model, the elimination of both tryptases is necessary to reveal the prominent roles of these serine proteases in joint inflammation and destruction. PMID: 18668540
3. TGF-beta stimulates mmcp-7 transcription through the Smad3-Smad4 pathway as well as c-fos induction PMID: 16730810
蛋白家族：

Peptidase S1 family, Tryptase subfamily
數據庫鏈接：

KEGG: mmu:100503895

STRING: 10090.ENSMUSP00000120741

UniGene: Mm.3301