Mouse Lysozyme,LZM ELISA Kit

產品詳情

文獻引用 (4) 說明書下載

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產品描述：

小鼠溶菌酶(LZM)酶聯免疫試劑盒（CSB-E11329m）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的LZM含量。LZM（溶菌酶）它是一種天然抗菌物質，廣泛存在于多種生物體內。研究機制上，其能特異性地作用于細菌細胞壁肽聚糖，破壞細胞壁結構，導致細菌溶解死亡，在醫藥、食品等領域有潛在應用價值。試劑盒檢測范圍為0.78 ng/mL-50 ng/mL，該產品可廣泛應用于小鼠感染性疾病模型、免疫系統功能評估、炎癥機制研究以及藥物干預實驗中溶菌酶動態變化的檢測，為科研人員探究先天免疫應答機制提供可靠工具。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
縮寫：

LZM
種屬：

Mus musculus (Mouse)
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

0.78 ng/mL-50 ng/mL
靈敏度：

0.195 ng/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Immunology
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of mouse LZM in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:100	Average %	93
	Range %	89-98
1:200	Average %	97
	Range %	94-101
1:400	Average %	98
	Range %	93-103
1:800	Average %	93
	Range %	87-100

回收率：

The recovery of mouse LZM spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	94	89-100
EDTA plasma (n=4)	91	85-98

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.