Mouse L-amino-acid oxidase(IL4I1) ELISA kit

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中文名稱：

小鼠L氨基酸氧化酶(IL4I1)酶聯(lián)免疫試劑盒
貨號：

CSB-EL011660MO
規(guī)格：

96T/48T
價格：

￥3600/￥2500
其他：

CUSABIO Wins 2024 Bioz Rapid Star? Award in the ELISA category

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產(chǎn)品描述：

小鼠L氨基酸氧化酶(IL4I1)酶聯(lián)免疫試劑盒（CSB-EL011660MO）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿、細(xì)胞裂解物樣本中的IL4I1含量。IL4I1靶點在相關(guān)疾病研究中備受關(guān)注。它具有獨特的生物學(xué)背景，在免疫調(diào)節(jié)等生理過程里發(fā)揮作用。研究機制方面，多聚焦于其參與的信號通路以及與疾病發(fā)生發(fā)展的關(guān)聯(lián)，為開發(fā)針對性治療手段、攻克疑難病癥提供理論依據(jù)。試劑盒檢測范圍為15.6 pg/mL-1000 pg/mL，科研人員可將其應(yīng)用于免疫學(xué)基礎(chǔ)研究、腫瘤模型構(gòu)建、炎癥性疾病機制探索等領(lǐng)域，例如評估不同病理狀態(tài)下IL4I1在血液或組織中的表達(dá)水平，或通過體外細(xì)胞實驗分析其功能調(diào)控網(wǎng)絡(luò)，為揭示IL4I1在免疫代謝中的分子機制提供可靠工具。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
別名：

Il4i1 ELISA Kit; Fig1 ELISA Kit; L-amino-acid oxidase ELISA Kit; LAAO ELISA Kit; LAO ELISA Kit; EC 1.4.3.2 ELISA Kit; Interleukin-4-induced protein 1 ELISA Kit; IL4-induced protein 1 ELISA Kit; Protein Fig-1 ELISA Kit; mFIG1 ELISA Kit
縮寫：

IL4I1
Uniprot No.：

O09046
種屬：

Mus musculus (Mouse)
樣本類型：

serum, plasma, tissue homogenates, cell lysates
檢測范圍：

15.6 pg/mL-1000 pg/mL
靈敏度：

3.9 pg/mL
反應(yīng)時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領(lǐng)域：

Cell Biology
測定原理：

quantitative
測定方法：

Sandwich

精密度：

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of mouse IL4I1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	101
	Range %	92-105
1:2	Average %	94
	Range %	90-99
1:4	Average %	95
	Range %	87-98
1:8	Average %	92
	Range %	85-95

回收率：

The recovery of mouse IL4I1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	103	94-107
EDTA plasma (n=4)	98	90-102

標(biāo)準(zhǔn)曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
1000	1.780	1.814	1.797	1.596
500	1.316	1.301	1.309	1.108
250	0.954	0.939	0.947	0.746
125	0.716	0.732	0.724	0.523
62.5	0.488	0.498	0.493	0.292
31.2	0.406	0.400	0.403	0.202
15.6	0.306	0.294	0.300	0.099
0	0.203	0.199	0.201

數(shù)據(jù)處理：

ELISA操作視頻及標(biāo)曲繪制軟件下載
貨期：

3-5 working days

引用文獻(xiàn)

Central memory CD4+ T cells play a protective role against immune checkpoint inhibitor-associated myocarditis J Yu,Cardiovascular research,2024
IL4I1 Is a Metabolic Immune Checkpoint that Activates the AHR and Promotes Tumor Progression A Sadik,Cell,2020

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功能：

Secreted L-amino-acid oxidase that acts as a key immunoregulator. Has preference for L-aromatic amino acids: converts phenylalanine (Phe), tyrosine (Tyr) and tryptophan (Trp) to phenylpyruvic acid (PP), hydroxyphenylpyruvic acid (HPP), and indole-3-pyruvic acid (I3P), respectively. Also has weak L-arginine oxidase activity. Acts as a negative regulator of anti-tumor immunity by mediating Trp degradation via an indole pyruvate pathway that activates the transcription factor AHR. IL4I1-mediated Trp catabolism generates I3P, giving rise to indole metabolites (indole-3-acetic acid (IAA) and indole-3-aldehyde (I3A)) and kynurenic acid, which act as ligands for AHR, a ligand-activated transcription factor that plays important roles in immunity and cancer. AHR activation by indoles following IL4I1-mediated Trp degradation enhances tumor progression by promoting cancer cell motility and suppressing adaptive immunity. Also has an immunoregulatory function in some immune cell, probably by mediating Trp degradation and promoting downstream AHR activation: inhibits T-cell activation and proliferation, promotes the differentiation of naive CD4(+) T-cells into FOXP3(+) regulatory T-cells (Treg) and regulates the development and function of B-cells. Also regulates M2 macrophage polarization by inhibiting T-cell activation. Also has antibacterial properties by inhibiting growth of Gram negative and Gram positive bacteria through the production of NH4(+) and H2O2.
基因功能參考文獻(xiàn)：
1. Data showed proteome changes in alveolar epithelial cells type II exposed to an infection with A. fumigatus with IL4I1 showing the most prominent increase in abundance both, in terms of relative abundance as well as activity. Also, in the infected lungs, there was levels of IL4I1 metabolic products. PMID: 28951444
亞細(xì)胞定位：

Secreted. Cytoplasmic vesicle, secretory vesicle, acrosome.; [Isoform 1]: Lysosome.; [Isoform 2]: Lysosome.
蛋白家族：

Flavin monoamine oxidase family, FIG1 subfamily
組織特異性：

Primarily found in immune tissues.; [Isoform 1]: Primarily found in immune tissues, mostly in B-lymphocytes.; [Isoform 2]: Restricted to the testis, predominantly in Sertoli cells at the periphery of the ducts, and the brain, including Purkinje cells, hip
數(shù)據(jù)庫鏈接：

KEGG: mmu:100328588

STRING: 10090.ENSMUSP00000113726

UniGene: Mm.2565

Mouse L-amino-acid oxidase(IL4I1) ELISA kit

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