Mouse Interleukin 31(IL-31) ELISA Kit

Mouse Interleukin 31(IL-31) ELISA Kit

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中文名稱：

小鼠白介素31(IL-31)酶聯(lián)免疫試劑盒
貨號(hào)：

CSB-E13660m
規(guī)格：

96T/48T
價(jià)格：

￥3600/￥2500
其他：

CUSABIO Wins 2024 Bioz Rapid Star? Award in the ELISA category

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產(chǎn)品描述：

小鼠白介素31(IL-31)酶聯(lián)免疫試劑盒（CSB-E13660m）為雙抗夾心法ELISA試劑盒，定量檢測(cè)血清、血漿、組織培養(yǎng)上清液、組織勻漿樣本中的IL31含量。IL31，即白介素31，是一種炎癥性細(xì)胞因子，主要由Th2細(xì)胞產(chǎn)生，通過(guò)IL31RA和OSMR受體復(fù)合物激活STAT3和STAT5等信號(hào)通路，參與皮膚免疫和炎癥性疾病，如特應(yīng)性皮炎。其作用機(jī)制涉及皮膚屏障、神經(jīng)生長(zhǎng)和瘙癢調(diào)節(jié)。試劑盒檢測(cè)范圍為31.25 pg/mL-2000 pg/mL，適用于科研領(lǐng)域探究IL-31在炎癥模型、免疫調(diào)控實(shí)驗(yàn)中的動(dòng)態(tài)表達(dá)，或用于評(píng)估藥物干預(yù)對(duì)IL-31分泌的影響，可為皮膚病學(xué)、免疫學(xué)等基礎(chǔ)研究提供可靠數(shù)據(jù)支持。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說(shuō)明書。
別名：

Il31Interleukin-31 ELISA Kit; IL-31 ELISA Kit
縮寫：

IL31
Uniprot No.：

Q6EAL8
種屬：

Mus musculus (Mouse)
樣本類型：

serum, plasma, cell culture supernates, tissue homogenates
檢測(cè)范圍：

31.25 pg/mL-2000 pg/mL
靈敏度：

7.81 pg/mL
反應(yīng)時(shí)間：

1-5h
樣本體積：

50-100ul
檢測(cè)波長(zhǎng)：

450 nm
研究領(lǐng)域：

Immunology
測(cè)定原理：

quantitative
測(cè)定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of mouse IL-31 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	90
	Range %	82-99
1:2	Average %	97
	Range %	87-105
1:4	Average %	95
	Range %	88-100
1:8	Average %	90
	Range %	82-98

回收率：

The recovery of mouse IL-31 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	97	93-104
EDTA plasma (n=4)	99	90-106

標(biāo)準(zhǔn)曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
2000	2.886	2.846	2.866	2.702
1000	2.347	2.336	2.342	2.178
500	1.559	1.602	1.581	1.417
250	0.897	0.878	0.888	0.724
125	0.532	0.557	0.545	0.381
62.5	0.366	0.374	0.370	0.206
31.25	0.262	0.245	0.254	0.090
0	0.162	0.165	0.164

數(shù)據(jù)處理：

ELISA操作視頻及標(biāo)曲繪制軟件下載
貨期：

3-5 working days

功能：

Activates STAT3 and possibly STAT1 and STAT5 through the IL31 heterodimeric receptor composed of IL31RA and OSMR. May function in skin immunity. Enhances myeloid progenitor cell survival in vitro. Induces RETNLA and serum amyloid A protein expression in macrophages.
基因功能參考文獻(xiàn)：
1. The serum levels of IL-33 and IL-31 were significantly up-regulated in both AR and allergic asthma patients compared with normal individuals. PMID: 30301503
2. EPAS1 links DOCK8 deficiency to atopic skin inflammation via IL-31 induction in CD4-positive T cells. PMID: 28067314
3. IL-31 increased the concentration of intracellular Ca(2+) ions in mouse keratinocytes. PMID: 29477033
4. IL-31 elicits itch not only through direct action on primary sensory neurones, but also by inducing LTB4 production in keratinocytes. PMID: 28512667
5. IL31 and its receptor, IL31RA, are highly expressed in various human and mouse cancer cell lines, as well as in tumor specimens from cancer patients. MC38 murine colon carcinoma cells depleted of IL31 exhibit an increase in invasive and migratory properties in vitro. PMID: 28147314
6. Oncostatin M and interleukin-31: Cytokines, receptors, signal transduction and physiology. PMID: 26198770
7. IL-31 upregulates IL-31RA expression in DRG neuron cell bodies, and cutaneous-injected IL-31-induced itching is enhanced by DRG IL-31RA expression in mice PMID: 25381841
8. our results suggest a functional cooperation between NFAT1 and JunB in mediating IL-31 gene expression in CD4(+) T cells PMID: 25595785
9. Data not only illustrate various functions that cysteines perform during IL-31 biosynthesis and secretion, but also highlight their potential roles in cytokine effector functions. PMID: 21182835
10. IL-31 may be involved in promoting the dermatitis and epithelial responses that characterize allergic and non-allergic diseases PMID: 15184896
11. IL-31 interaction with IL-31 receptor (IL-31R) is identified in an acute model of T helper cell type 2 inflammation in the lung. PMID: 17353366
12. -31 antibody reduces scratching behaviour in an atopic dermatitis-like murine model during the onset of clinical skin manifestations PMID: 19054054
13. these data provide the first evidence of a function for IL-31-IL-31R interactions in limiting the magnitude of type 2 inflammatory responses within the intestine. PMID: 19414760
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亞細(xì)胞定位：

Secreted.
數(shù)據(jù)庫(kù)鏈接：

KEGG: mmu:76399

STRING: 10090.ENSMUSP00000031389

UniGene: Mm.75049

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.