Mouse Interleukin-19(IL19) ELISA kit

說明書下載

中文名稱：

小鼠白介素19(IL19)酶聯免疫試劑盒
貨號：

CSB-EL011612MO
規格：

96T/48T
價格：

￥3600/￥2500
促銷：
其他：

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小鼠白介素19(IL19)酶聯免疫試劑盒（CSB-EL011612MO）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的IL19含量。IL19 屬 IL-10 細胞因子家族成員。其在炎癥與免疫調節等方面發揮重要作用。研究機制上，它能結合特異性受體，激活下游信號通路，參與調控細胞的增殖、分化以及炎癥因子的釋放，目前是相關疾病治療靶點研究的熱點。試劑盒檢測范圍為12.5 pg/mL-800 pg/mL，支持科研領域對小鼠模型中IL19表達動態的研究，例如炎癥性疾病機制探索、藥物干預效果評估或免疫調控通路分析，為體外實驗提供可靠的定量數據支持，但不適用于臨床診斷或體內檢測用途。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
別名：

Il19Interleukin-19 ELISA Kit; IL-19 ELISA Kit
縮寫：

IL19
Uniprot No.：

Q8CJ70
種屬：

Mus musculus (Mouse)
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

12.5 pg/mL-800 pg/mL
靈敏度：

3.12 pg/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Immunology
測定原理：

quantitative
測定方法：

Sandwich

精密度：

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of mouse IL19 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	107
	Range %	96-112
1:2	Average %	103
	Range %	94-107
1:4	Average %	95
	Range %	86-99
1:8	Average %	100
	Range %	90-104

回收率：

The recovery of mouse IL19 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	102	88-106
EDTA plasma (n=4)	103	92-107

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
800	2.707	2.656	2.682	2.542
400	1.494	1.544	1.519	1.379
200	0.829	0.845	0.837	0.697
100	0.422	0.446	0.434	0.294
50	0.309	0.313	0.311	0.171
25	0.201	0.212	0.207	0.067
12.5	0.176	0.171	0.174	0.034
0	0.142	0.138	0.140

數據處理：

ELISA操作視頻及標曲繪制軟件下載
貨期：

3-5 working days

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功能：

May play some important roles in inflammatory responses. Up-regulates IL-6 and TNF-alpha and induces apoptosis.
基因功能參考文獻：
1. Data, including data from studies using knockout mice, suggest that interleukin-19 suppresses hapten-dependent skin inflammation in elicitation phase of contact hypersensitivity. PMID: 29386478
2. Attenuated colitis in IL-19-deficient animals was associated with reduced numbers of IL-6-producing macrophages in the inflamed colonic lamina propria. Microbial-driven expression of IL-19 by intestinal macrophages may contribute to the pathogenesis of inflammatory bowel disease. PMID: 28864472
3. IL-19 can promote angiogenesis in the absence of hypoxia by at least two distinct mechanisms: 1) direct effects on vascular cells and 2) indirect effects by stimulation of macrophages. PMID: 27053520
4. IL-19 plays an anti-inflammatory role in the Th2-mediated colitis model. PMID: 27942772
5. microglia may self-limit their inflammatory response by producing the negative regulator IL-19. PMID: 25794104
6. Lack of IL-19 exacerbates intimal hyperplasia PMID: 24814101
7. we delineate the detailed molecular pathway for IL-4 up-regulation of IL-19 in keratinocytes, which may play an important role in AD pathogenesis. PMID: 24943510
8. Il-19 expression is increased in astrocytes following bacterial challenge. PMID: 24677051
9. IL-19 is important for cutaneous wound healing because it upregulates KGF expression. PMID: 23582717
10. IL-19 is a potent inhibitor of experimental atherosclerosis. PMID: 23950143
11. IL-19 is pivotal in the pathogenesis of breast cancer. PMID: 22186257
12. endogenous IL-19 is a constituent of the regulome that controls both processes in vivo PMID: 22110701
13. IL-19 drives pathogenic innate immune responses in the colon. PMID: 19834971
14. In a dust mite-induced murine model of asthma, the IL-19 level in asthmatic BALB/cJ mice is twice that of healthy control mice. PMID: 15557163
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亞細胞定位：

Secreted.
蛋白家族：

IL-10 family
數據庫鏈接：

KEGG: mmu:329244

STRING: 10090.ENSMUSP00000016668

UniGene: Mm.131480

Mouse Interleukin-19(IL19) ELISA kit

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