Mouse Interleukin-19(IL19) ELISA kit

說明書下載

中文名稱：

小鼠白介素19(IL19)酶聯(lián)免疫試劑盒
貨號：

CSB-EL011612MO
規(guī)格：

96T/48T
價格：

￥3600/￥2500
其他：

CUSABIO Wins 2024 Bioz Rapid Star? Award in the ELISA category

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產(chǎn)品描述：

小鼠白介素19(IL19)酶聯(lián)免疫試劑盒（CSB-EL011612MO）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的IL19含量。IL19 屬 IL-10 細(xì)胞因子家族成員。其在炎癥與免疫調(diào)節(jié)等方面發(fā)揮重要作用。研究機(jī)制上，它能結(jié)合特異性受體，激活下游信號通路，參與調(diào)控細(xì)胞的增殖、分化以及炎癥因子的釋放，目前是相關(guān)疾病治療靶點研究的熱點。試劑盒檢測范圍為12.5 pg/mL-800 pg/mL，支持科研領(lǐng)域?qū)π∈竽Ｐ椭蠭L19表達(dá)動態(tài)的研究，例如炎癥性疾病機(jī)制探索、藥物干預(yù)效果評估或免疫調(diào)控通路分析，為體外實驗提供可靠的定量數(shù)據(jù)支持，但不適用于臨床診斷或體內(nèi)檢測用途。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
別名：

Il19Interleukin-19 ELISA Kit; IL-19 ELISA Kit
縮寫：

IL19
Uniprot No.：

Q8CJ70
種屬：

Mus musculus (Mouse)
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

12.5 pg/mL-800 pg/mL
靈敏度：

3.12 pg/mL
反應(yīng)時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領(lǐng)域：

Immunology
測定原理：

quantitative
測定方法：

Sandwich

精密度：

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of mouse IL19 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	107
	Range %	96-112
1:2	Average %	103
	Range %	94-107
1:4	Average %	95
	Range %	86-99
1:8	Average %	100
	Range %	90-104

回收率：

The recovery of mouse IL19 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	102	88-106
EDTA plasma (n=4)	103	92-107

標(biāo)準(zhǔn)曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
800	2.707	2.656	2.682	2.542
400	1.494	1.544	1.519	1.379
200	0.829	0.845	0.837	0.697
100	0.422	0.446	0.434	0.294
50	0.309	0.313	0.311	0.171
25	0.201	0.212	0.207	0.067
12.5	0.176	0.171	0.174	0.034
0	0.142	0.138	0.140

數(shù)據(jù)處理：

ELISA操作視頻及標(biāo)曲繪制軟件下載
貨期：

3-5 working days

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功能：

May play some important roles in inflammatory responses. Up-regulates IL-6 and TNF-alpha and induces apoptosis.
基因功能參考文獻(xiàn)：
1. Data, including data from studies using knockout mice, suggest that interleukin-19 suppresses hapten-dependent skin inflammation in elicitation phase of contact hypersensitivity. PMID: 29386478
2. Attenuated colitis in IL-19-deficient animals was associated with reduced numbers of IL-6-producing macrophages in the inflamed colonic lamina propria. Microbial-driven expression of IL-19 by intestinal macrophages may contribute to the pathogenesis of inflammatory bowel disease. PMID: 28864472
3. IL-19 can promote angiogenesis in the absence of hypoxia by at least two distinct mechanisms: 1) direct effects on vascular cells and 2) indirect effects by stimulation of macrophages. PMID: 27053520
4. IL-19 plays an anti-inflammatory role in the Th2-mediated colitis model. PMID: 27942772
5. microglia may self-limit their inflammatory response by producing the negative regulator IL-19. PMID: 25794104
6. Lack of IL-19 exacerbates intimal hyperplasia PMID: 24814101
7. we delineate the detailed molecular pathway for IL-4 up-regulation of IL-19 in keratinocytes, which may play an important role in AD pathogenesis. PMID: 24943510
8. Il-19 expression is increased in astrocytes following bacterial challenge. PMID: 24677051
9. IL-19 is important for cutaneous wound healing because it upregulates KGF expression. PMID: 23582717
10. IL-19 is a potent inhibitor of experimental atherosclerosis. PMID: 23950143
11. IL-19 is pivotal in the pathogenesis of breast cancer. PMID: 22186257
12. endogenous IL-19 is a constituent of the regulome that controls both processes in vivo PMID: 22110701
13. IL-19 drives pathogenic innate immune responses in the colon. PMID: 19834971
14. In a dust mite-induced murine model of asthma, the IL-19 level in asthmatic BALB/cJ mice is twice that of healthy control mice. PMID: 15557163
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亞細(xì)胞定位：

Secreted.
蛋白家族：

IL-10 family
數(shù)據(jù)庫鏈接：

KEGG: mmu:329244

STRING: 10090.ENSMUSP00000016668

UniGene: Mm.131480

Mouse Interleukin-19(IL19) ELISA kit

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