Mouse Insulin-like peptide INSL5(INSL5) ELISA kit

Mouse Insulin-like peptide INSL5(INSL5) ELISA kit

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中文名稱：

小鼠胰島素樣肽INSL5(INSL5)酶聯免疫試劑盒
貨號：

CSB-EL011749MO
規格：

96T/48T
價格：

￥3600/￥2500
其他：

CUSABIO Wins 2024 Bioz Rapid Star? Award in the ELISA category

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產品描述：

小鼠胰島素樣肽INSL5(INSL5)酶聯免疫試劑盒（CSB-EL011749MO）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織培養上清液、組織勻漿樣本中的INSL5含量。INSL5，即胰島素樣肽5，是胰島素超家族的一員，具有胰島素家族的經典結構。其研究機制主要涉及與受體RXFP4的相互作用，調節能量代謝、食欲調節和腸道蠕動等生理功能。近期研究發現，INSL5在腫瘤糖代謝調控中發揮重要作用，通過激活STAT5信號通路促進糖酵解相關基因的表達，影響細胞糖代謝重編程，進而促進腫瘤進展。試劑盒檢測范圍為3.12 pg/mL-200 pg/mL，可為研究INSL5在代謝綜合征、食欲異常調控、胃腸道功能紊亂等領域的分子機制提供可靠工具，也可用于評估藥物干預或基因修飾模型中INSL5的表達變化。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
別名：

Insl5 ELISA Kit; Rif ELISA Kit; Rif2 ELISA Kit; Zins3 ELISA Kit; Insulin-like peptide INSL5 ELISA Kit; Insulin-like peptide 5 ELISA Kit; Relaxin/insulin-like factor 2 ELISA Kit; Relaxin/insulin-like protein) [Cleaved into: Insulin-like peptide INSL5 B chain; Insulin-like peptide INSL5 A chain] ELISA Kit
縮寫：

INSL5
Uniprot No.：

Q9WUG6
種屬：

Mus musculus (Mouse)
樣本類型：

serum, plasma, cell culture supernates, tissue homogenates
檢測范圍：

3.12 pg/mL-200 pg/mL
靈敏度：

0.78 pg/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Signal Transduction
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of mouse INSL in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:100	Average %	93
	Range %	90-96
1:200	Average %	100
	Range %	97-103
1:400	Average %	88
	Range %	85-93
1:800	Average %	107
	Range %	103-110

回收率：

The recovery of mouse INSL spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	84	81-89
EDTA plasma (n=4)	98	93-102

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
200	2.417	2.446	2.432	2.262
100	2.046	2.008	2.027	1.857
50	1.562	1.568	1.565	1.395
25	1.017	1.035	1.026	0.856
12.5	0.692	0.669	0.681	0.511
6.25	0.425	0.435	0.430	0.260
3.12	0.306	0.330	0.318	0.148
0	0.169	0.171	0.170

數據處理：

ELISA操作視頻及標曲繪制軟件下載
貨期：

3-5 working days

功能：

May have a role in gut contractility or in thymic development and regulation. Activates RXFP4 with high potency and appears to be the endogenous ligand for this receptor.
基因功能參考文獻：
1. Mass spectral analysis of purified enteroendocrine cells and tissue homogenates identified the exact sequence of A and B chains of INSL5 endogenously expressed in L-cells. PMID: 28857318
2. Insl5 is an orexigenic hormone released from colonic L-cells, which promotes appetite during conditions of energy deprivation. PMID: 25028498
3. INSL5 may be a unique marker of colorectal enteroendocrine cells, and INSL5-RXFP4 signaling might play a role in an autocrine/paracrine fashion in the colorectal epithelium and rectal neuroendocrine tumors. PMID: 23438439
4. Insl5 knockout mice displayed impaired male and female fertility that are due to marked reduction in sperm motility and irregular length of the estrous cycle. Also impaired glucose homeostasis due to glucose intolerance. PMID: 22822165
5. Insulin-like peptide 5 is expressed in mouse brain and has a role in mobilization of calcium PMID: 16601133
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亞細胞定位：

Secreted.
蛋白家族：

Insulin family
組織特異性：

Highest expression in colon with lower levels in thymus. Minimal levels in testis.
數據庫鏈接：

KEGG: mmu:23919

STRING: 10090.ENSMUSP00000081416

UniGene: Mm.100163

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.