Mouse Fibrinogen-like protein 1(FGL1) ELISA kit
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中文名稱：小鼠纖維蛋白原樣蛋白1(FGL1)酶聯(lián)免疫試劑盒
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貨號(hào)：CSB-EL008653MO
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規(guī)格：96T/48T
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價(jià)格：￥3600/￥2500
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其他：
產(chǎn)品詳情
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產(chǎn)品描述：小鼠纖維蛋白原樣蛋白1(FGL1)酶聯(lián)免疫試劑盒（CSB-EL008653MO）為競爭法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的FGL1含量。FGL1是一種在腫瘤細(xì)胞和肝細(xì)胞中表達(dá)的免疫檢查點(diǎn)分子，其表達(dá)在多種腫瘤中上調(diào)，與抗PD-L1/PD-1治療的耐藥性和預(yù)后不良有關(guān)。研究揭示FGL1通過作為LAG-3的主要配體抑制T細(xì)胞活化，其乙酰化修飾和泛素化降解調(diào)控腫瘤免疫逃逸。試劑盒檢測范圍為31.25 pg/mL-2000 pg/mL，適用于基礎(chǔ)科研中探究肝臟代謝功能、免疫調(diào)節(jié)機(jī)制、腫瘤微環(huán)境等領(lǐng)域的FGL1動(dòng)態(tài)表達(dá)研究，為動(dòng)物模型構(gòu)建、藥物干預(yù)效果評(píng)估及分子機(jī)制解析提供可靠的數(shù)據(jù)支持本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
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別名：Fgl1 ELISA Kit; Mfire1 ELISA Kit; Fibrinogen-like protein 1 ELISA Kit; Fibrinogen-related protein 1 ELISA Kit; Mfrep-1 ELISA Kit; Liver fibrinogen-related protein-1 ELISA Kit; Mfire-1 ELISA Kit
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縮寫：FGL1
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Uniprot No.：
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種屬：Mus musculus (Mouse)
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樣本類型：serum, plasma, tissue homogenates
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檢測范圍：31.25 pg/mL-2000 pg/mL
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靈敏度：7.8 pg/mL
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反應(yīng)時(shí)間：1-5h
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樣本體積：50-100ul
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檢測波長：450 nm
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研究領(lǐng)域：Cardiovascular
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測定原理：quantitative
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測定方法：Competitive
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精密度：
Intra-assay Precision (Precision within an assay): CV%<8% Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV%<10% Three samples of known concentration were tested in twenty assays to assess. -
線性度：
To assess the linearity of the assay, samples were spiked with high concentrations of mouse FGL1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. Sample Serum(n=4) 1:100 Average % 95 Range % 91-99 1:200 Average % 84 Range % 81-89 1:400 Average % 83 Range % 90-96 1:800 Average % 87 Range % 84-90 -
回收率：
The recovery of mouse FGL1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. Sample Type Average % Recovery Range Serum (n=5) 104 100-108 EDTA plasma (n=4) 107 104-110 -
標(biāo)準(zhǔn)曲線：
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. 
pg/ml OD1 OD2 Average 2000 0.076 0.079 0.078 1000 0.135 0.146 0.141 500 0.206 0.220 0.213 250 0.362 0.379 0.371 125 0.593 0.586 0.590 62.5 0.901 0.932 0.917 31.25 1.213 1.236 1.225 0 2.054 2.098 2.076 -
數(shù)據(jù)處理：
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貨期：3-5 working days
引用文獻(xiàn)
- FBXO38 mediates FGL1 ubiquitination and degradation to enhance cancer immunity and suppress inflammation T Tian,Cell reports,2023
- Plasma Fibrinogen-Like 1 as a Potential Biomarker for Radiation-Induced Liver Injury Han NK, et al,Cells,2019
- A novel hepatokine, HFREP1, plays a crucial role in the development of insulin resistance and type 2 diabetes Wu HT.et al,Diabetologia.,2016
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相關(guān)產(chǎn)品
靶點(diǎn)詳情
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功能：Immune suppressive molecule that inhibits antigen-specific T-cell activation by acting as a major ligand of LAG3. Responsible for LAG3 T-cell inhibitory function. Binds LAG3 independently from MHC class II (MHC-II). Secreted by, and promotes growth of, hepatocytes.
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基因功能參考文獻(xiàn)：
- The results suggest that Fgl1 acts as a tumor suppressor in hepatocellular cancer through an Akt-dependent mechanism and support its role as a potential therapeutic target in hepatocellular carcinoma. PMID: 26225745
- Hepassocin plays an important role in non-alcoholic fatty liver disease and induces hepatic lipid accumulation through an ERK1/2-dependent pathway. PMID: 23792031
- Fgl1 is a member of an emerging group of proteins with key roles in metabolism and liver regeneration. PMID: 23483972
- HNF1 binding site and HNF1alpha are critical to liver-specific expression of HPS, and down-regulation or loss of HNF1alpha causes, at least in part, the transcriptional down-regulation of HPS in HCC. PMID: 19304666
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亞細(xì)胞定位：Secreted.
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組織特異性：Mainly expressed in liver. Also expressed in brown adipose tissue.
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數(shù)據(jù)庫鏈接：
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