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中文名稱：小鼠上皮中性粒細胞活化肽78(ENA-78/CXCL5)酶聯(lián)免疫試劑盒
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貨號：CSB-E08180m
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規(guī)格：96T/48T
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價格：￥3200/￥2500
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促銷：
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其他：
產(chǎn)品詳情
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產(chǎn)品描述：小鼠上皮中性粒細胞活化肽78(ENA-78/CXCL5)酶聯(lián)免疫試劑盒（CSB-E08180m）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的CXCL5含量。CXCL5是一種趨化因子，在腫瘤微環(huán)境、炎癥反應(yīng)等方面有重要作用。研究表明，它可招募特定免疫細胞，參與腫瘤血管生成、侵襲轉(zhuǎn)移等過程。以CXCL5為靶點，有望從調(diào)控其表達、阻斷相關(guān)信號通路等方面開發(fā)治療策略。試劑盒檢測范圍為62.5 pg/ml- 4000 pg/ml，靈敏度為15.6 pg/ml。適用于炎癥機制研究、疾病模型（如肺炎、關(guān)節(jié)炎或腫瘤模型）中趨化因子動態(tài)變化的分析，以及藥物干預(yù)實驗中生物標(biāo)志物的定量評估。科研人員可通過該產(chǎn)品探究CXCL5在免疫調(diào)控、病理進程及潛在治療靶點中的功能，為相關(guān)基礎(chǔ)研究提供可靠工具。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
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別名：Cxcl5 ELISA Kit; Scyb5C-X-C motif chemokine 5 ELISA Kit; Cytokine LIX ELISA Kit; Small-inducible cytokine B5) [Cleaved into: GCP-2(1-78); GCP-2(9-78)] ELISA Kit
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縮寫：
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Uniprot No.：
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種屬：Mus musculus (Mouse)
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樣本類型：serum, plasma, tissue homogenates
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檢測范圍：62.5 pg/ml - 4000 pg/ml
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靈敏度：15.6 pg/ml
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反應(yīng)時間：1-5h
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樣本體積：50-100ul
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檢測波長：450 nm
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研究領(lǐng)域：Immunology
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測定原理：quantitative
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測定方法：Sandwich
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精密度：
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Intra-Assay Precision
Inter-Assay Precision
Sample
1
2
3
1
2
3
n
20
20
20
20
20
20
Mean(pg/ml)
516.646
497.903
491.373
484.723
483.883
477.090
SD
0.035
0.033
0.038
0.041
0.041
0.038
CV(%)
7.172
6.286
7.350
8.055
8.039
7.600
TYPICAL DATA
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml
OD1
OD2
Average
Corrected
4000
2.88
2.876
2.878
2.726
2000
2.239
2.235
2.237
2.085
1000
1.263
1.259
1.261
1.109
500
0.646
0.642
0.644
0.492
250
0.342
0.338
0.340
0.188
125
0.249
0.245
0.247
0.095
62.5
0.218
0.214
0.216
0.064
0.064
0
0.154
0.150
0.152
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線性度：
To assess the linearity of the assay, samples were spiked with high concentrations of mouse ENA-78/CXCL5 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
Sample
Serum(n=4)
1:20
Average %
88
Range %
83-91
1:40
Average %
104
Range %
98-108
1:80
Average %
92
Range %
87-96
1:160
Average %
96
Range %
91-99
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回收率：
The recovery of mouse ENA-78/CXCL5 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type
Average % Recovery
Range%
Serum (n=5)
90
84-94
EDTA plasma (n=4)
94
87-97
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數(shù)據(jù)處理：
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貨期：3-5 working days
引用文獻
- Astrocytic CXCL5 hinders microglial phagocytosis of myelin debris and aggravates white matter injury in chronic cerebral ischemia Q Cao,Journal of neuroinflammation,2023
產(chǎn)品評價
樣品類型：細胞培養(yǎng)上清液
樣品信息：小鼠
稀釋比：沒有稀釋
產(chǎn)品評價： 我用CSB-E08180m檢測正常細胞上清及il-17a干預(yù)后細胞上清，標(biāo)準(zhǔn)曲線的OD值為3.3675-0.064，樣本的OD值區(qū)間為0.076-0.183，檢測過程較為清晰，結(jié)果準(zhǔn)確。
By 心老師
相關(guān)產(chǎn)品
靶點詳情
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功能：May participate in the recruitment of inflammatory cells by injured or infected tissue. GCP-2(1-78) and, more potent, GCP-2(9-78) attract neutrophils and are involved in neutrophil activation.
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基因功能參考文獻：
- Identify Cxcl5 as a novel target of AHR-mediated gene expression in primary mouse keratinocytes. PMID: 28973351
- IL-17RA regulates CXL-1 and 5 production in the lungs during the adaptive response. PMID: 26871571
- STAT3 is required for maximal OSM-induced CXCL5 expression. PMID: 25692402
- CXCL5 has a role in neutrophil recruitment in TH17-mediated glomerulonephritis PMID: 24904089
- Since adaptive villus growth occurs despite impaired CXCL5 expression and enhanced angiogenesis, this suggests that the growth of new blood vessels is not needed for resection-induced mucosal surface area expansion following massive SBR. PMID: 24888846
- CXCL5 regulates pulmonary responses to infection and plays a central role in conferring clock control of inflammation. PMID: 25064128
- findings demonstrated that CXCL1 and CXCL5 are increased in circulation with onset of T2D, are produced by islets under stress, and synergistically affect islet function, suggesting that these chemokines participate in pathogenesis of T2D. PMID: 24928936
- TLR2-induced epithelial-derived CXCL5 is critical for polymorphonuclear leukocyte-driven destructive inflammation in pulmonary tuberculosis. PMID: 24509076
- data suggest that CXCL6 contributes to experimental pulmonary fibrosis, and CXCL6 inhibition might be used to reduce lung toxicity associated with bleomycin treatment. PMID: 23975892
- Our data suggest that the differential regulation of the chemokine CXCL5 between osteoblasts and endothelial cells upon FGF2 treatment is involved in Hematopoietic stem cell mobilization from the osteoblast niche or bone marrow to peripheral blood. PMID: 22827607
- CXCL5 modulated macrophage activation, increased expression of the cholesterol efflux regulatory protein ABCA1, and enhanced cholesterol efflux activity in macrophages. PMID: 23376791
- The omentum is the main site of early neutrophil entry into the peritoneal cavity, where the action of CXCL5 synergizes with matrix metalloproteinases MMP-2 and -9 to promote neutrophil migration during the inflammatory process. PMID: 23225890
- CXCL5-dependent signaling cascades are essential for the recruitment of macrophages to the lungs upon subacute second hand smoke exposure. PMID: 22362385
- Cxcr2, Cxcl5, and commensal bacteria have critical roles in regulation of the IL-17/G-CSF axis and neutrophil homeostasis at mucosal sites PMID: 22326959
- In a mouse model of lung ischemia-induced angiogenesis, LIX predominates in eliciting a pro-angiogenic phenotype among the three ELR+ CXC chemokines (KC, LIX, and MIP-2) studied. PMID: 20144627
- regulates chemokine scavenging and pulmonary host defense to bacterial infection PMID: 20643340
- The villus growth observed in resection-induced adaptation is associated with increased expression of the chemokine CXCL5 within the lamina propria. PMID: 20471049
- LIX is expressed in response to muscle damage in vivo. Its spatial distribution in injured muscle suggests expression by satellite cells. PMID: 12077361
- Cloning and genomic localization of Scyb5. PMID: 12439624
- The PMN transendothelial migration induced by the activated myocytes is dependent on the production and secretion of the chemokines LIX and KC by the activated myocytes. PMID: 14988231
- proliferative effect of LIX is specific towards the primitive hematopoietic cells PMID: 15036238
- GCP-2 modulates cellular immunity in part through IL-8Rbeta interactions PMID: 15356099
- alveolar epithelial type II (AEII) cells are the primary source of CXCL5 in the rodent lung via TLR4-dependent signaling PMID: 15778492
- CXCR1 is a functional receptor for GCP-2/CXCL6 and interleukin-8/CXCL8 in mouse PMID: 17197447
- One of the mechanisms whereby endogenous IFN-gamma mitigates the manifestations of CIA consists of inhibiting production of GCP-2, thereby limiting mobilization and infiltration of neutrophils, which are important actors in joint inflammation. PMID: 17200147
- unveils a proinflammatory pathway centered on kinin B1 receptor activation of CXCL5 leading to leukocyte trafficking PMID: 17878384
- CXCL5, is ectopically expressed in the corneal epithelial cells of Dstn(corn1) mice, and targeting of the receptor for this chemokine inhibits neutrophil recruitment PMID: 18628996
- Secretion of CXCL5 by white adipose tissue resident macrophages represents a link between obesity, inflammation, and insulin resistance. PMID: 19356715
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亞細胞定位：Secreted.
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蛋白家族：Intercrine alpha (chemokine CxC) family
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