Mouse Epidermal growth factor-like protein 6(EGFL6) ELISA kit

產品詳情

說明書下載

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產品描述：

小鼠表皮生長因子樣蛋白6(EGFL6)酶聯免疫試劑盒（CSB-EL007475MO）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的EGFL6含量。EGFL6是一種重要靶點。它在細胞增殖、遷移等生理過程中發揮作用。研究發現其異常表達與多種疾病相關，如腫瘤的發生發展。科研人員正深入探究其作用機制，期望通過靶向EGFL6開發新的診斷方法和治療策略。試劑盒檢測范圍為93.75 pg/mL-6000 pg/mL，科研人員可通過探究EGFL6在小鼠模型中的功能特性，例如在腫瘤發生、血管異常增生或創傷修復等研究領域，定量分析不同實驗條件下EGFL6的動態變化，為疾病機制研究或藥物干預效果評估提供可靠數據支持。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
別名：

Egfl6 ELISA Kit; Maeg ELISA Kit; Epidermal growth factor-like protein 6 ELISA Kit; EGF-like protein 6 ELISA Kit; MAM and EGF domains-containing gene protein ELISA Kit; Protein W80 ELISA Kit
縮寫：

EGFL6
Uniprot No.：

Q9JJZ5
種屬：

Mus musculus (Mouse)
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

93.75 pg/mL-6000 pg/mL
靈敏度：

23.43 pg/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Signal Transduction
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of mouse EGFL6 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	90
	Range %	85-94
1:2	Average %	98
	Range %	94-101
1:4	Average %	102
	Range %	94-108
1:8	Average %	94
	Range %	87-97

回收率：

The recovery of mouse EGFL6 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	97	94-103
EDTA plasma (n=4)	93	89-96

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

功能：

May bind integrin alpha-8/beta-1 and play a role in hair follicle morphogenesis. Promotes matrix assembly.
基因功能參考文獻：
1. EGLF6 mediates a paracrine mechanism of cross-talk between vascular endothelial cells and osteoblasts PMID: 21531721
2. MAEG may play a role as a mediator regulating epithelial-mesenchymal interaction through binding to RGD-binding integrins including alpha8beta1 during hair follicle development. PMID: 15572035
亞細胞定位：

Secreted, extracellular space, extracellular matrix, basement membrane.
蛋白家族：

Nephronectin family
組織特異性：

Expressed at basement membrane of pelage follicles (at protein level).
數據庫鏈接：

KEGG: mmu:54156

UniGene: Mm.37707