Mouse Disintegrin and metalloproteinase domain-containing protein 33(ADAM33) ELISA kit

產品詳情

說明書下載

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產品描述：

小鼠解整合素樣金屬蛋白酶33(ADAM33)酶聯免疫試劑盒（CSB-EL001290MO）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的ADAM33含量。ADAM33是一種編碼解整合素和金屬蛋白酶的基因，其表達與哮喘、慢性阻塞性肺疾病和鼻炎等疾病相關。該基因通過參與細胞粘附、遷移和信號傳導過程，在多種生物過程中發揮作用，如受精、肌肉發育和神經發生。ADAM33基因的多態性與哮喘等疾病的易感性有關。試劑盒檢測范圍為15.6 pg/mL-1000 pg/mL，適用于基礎研究中ADAM33的表達水平分析，如探索肺部疾病模型中的分子調控機制、評估藥物干預對氣道重塑的影響，或驗證基因編輯動物模型中靶蛋白的表達變化。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
別名：

Adam33Disintegrin and metalloproteinase domain-containing protein 33 ELISA kit; ADAM 33 ELISA kit; EC 3.4.24.- ELISA kit
縮寫：

ADAM33
Uniprot No.：

Q923W9
種屬：

Mus musculus (Mouse)
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

15.6 pg/mL-1000 pg/mL
靈敏度：

3.9 pg/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Cell Biology
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of mouse ADAM33 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	93
	Range %	88-97
1:2	Average %	84
	Range %	80-88
1:4	Average %	95
	Range %	90-100
1:8	Average %	104
	Range %	99-109

回收率：

The recovery of mouse ADAM33 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	90	85-94
EDTA plasma (n=4)	92	87-96

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

基因功能參考文獻：
1. The mouse and human predicted proteins consisted of 797 and 813 amino acids, respectively, and they shared 70% homology of the entire amino acid sequence. PMID: 11814695
2. Identification and preliminary characterization of mouse Adam33. PMID: 11897009
3. ADAM33 is not essential for growth or reproduction in the mouse and does not modulate baseline or allergen-induced airway responsiveness. PMID: 16943435
4. Adam33/Adam33 shows 2 significant increments in expression during lung morphogenesis, suggesting important developmental regulation. PMID: 19665773
亞細胞定位：

Membrane; Single-pass type I membrane protein.
數據庫鏈接：

KEGG: mmu:110751

STRING: 10090.ENSMUSP00000105861

UniGene: Mm.108550